Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA 92507, U.S.A.
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA 94305, U.S.A.
Mol Plant Microbe Interact. 2021 Apr;34(4):439-447. doi: 10.1094/MPMI-11-20-0310-R. Epub 2021 Mar 26.
The induction of plant nutrient secretion systems is critical for successful pathogen infection. Some bacterial pathogens (e.g., spp.) use transcription activator-like (TAL) effectors to induce transcription of SWEET sucrose efflux transporters. pv. strain DC3000 lacks TAL effectors yet is able to induce multiple SWEETs in by unknown mechanisms. Because bacteria require other nutrients in addition to sugars for efficient reproduction, we hypothesized that spp. may depend on host transcription factors involved in secretory programs to increase access to essential nutrients. Bioinformatic analyses identified the basic-leucine zipper transcription factor bZIP11 as a potential regulator of nutrient transporters, including SWEETs and UmamiT amino acid transporters. Inducible downregulation of expression in resulted in reduced growth of pv. strain DC3000, whereas inducible overexpression of resulted in increased bacterial growth, supporting the hypothesis that bZIP11-regulated transcription programs are essential for maximal pathogen titer in leaves. Our data are consistent with a model in which a pathogen alters host transcription factor expression upstream of secretory transcription networks to promote nutrient efflux from host cells.[Formula: see text] Copyright © 2021 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
植物养分分泌系统的诱导对于成功的病原体感染至关重要。一些细菌病原体（例如， spp.）使用转录激活子样（TAL）效应子来诱导 SWEET 蔗糖外排转运蛋白的转录。 pv. 菌株 DC3000 缺乏 TAL 效应子，但能够通过未知机制在 中诱导多个 SWEET。由于细菌除了糖之外还需要其他营养物质才能有效地繁殖，我们假设 spp. 可能依赖于参与分泌程序的宿主转录因子来增加对必需营养物质的获取。生物信息学分析鉴定了 碱性亮氨酸拉链转录因子 bZIP11 作为潜在的营养转运蛋白调节剂，包括 SWEET 和鲜味氨基酸转运蛋白。 在 中诱导性下调 表达导致 pv. 菌株 DC3000 的生长减少，而 诱导性过表达 导致细菌生长增加，这支持了这样一种假设，即 bZIP11 调节的转录程序对于叶片中最大病原体滴度是必不可少的。我们的数据与一种模型一致，即病原体改变宿主转录因子表达，在分泌转录网络的上游促进养分从宿主细胞中流出。[公式：见正文]版权所有 © 2021 作者。这是一个在 CC BY-NC-ND 4.0 国际许可下分发的开放获取文章。
