College of Chemistry, Fu Zhou University, FuZhou, 350002, PR China; Institute of Glycobiochemistry, National Engineering Research Centre of Chemical Fertilizer Catalyst, Fu Zhou University, FuZhou, 350002, PR China.
College of Biological Science and Engineering, Fu Zhou University, FuZhou, 350002, PR China.
Carbohydr Res. 2021 Jan;499:108226. doi: 10.1016/j.carres.2020.108226. Epub 2021 Jan 2.
The O-sulfation, including 2-O- and 6-O-sulfation, in heparan sulfate (HS) have important biological and pathophysiological roles. Therefore, the ability to chemically generate a series of oligosaccharides, which have a similar structure to the naturally-occurring, 2-O- and 6-O-sulfating oligosaccharides from HS, would greatly contribute to investigating their natural role in HS. In this study, a heparin oligosaccharide library, including dp2, dp4 and dp6, were prepared from the chemical modification of the fully sulfated dp2, dp4 and dp6. Chemical reaction conditions were optimized to generate different patterns of 2-O- and 6-O-sulfated oligosaccharides, then the disaccharide composition and structure of the library was detected by high-performance liquid chromatography-ion trap/time-of-flight mass spectrometry (LC-IT-TOF/MS) analysis. This provides a foundation for further structural and functional studies of O-sulfated groups in HS.
O-硫酸化，包括 2-O-硫酸化和 6-O-硫酸化，在肝素硫酸酯 (HS) 中具有重要的生物学和病理生理学作用。因此，能够通过化学方法生成一系列具有类似结构的寡糖，这些寡糖类似于 HS 中天然存在的 2-O-硫酸化和 6-O-硫酸化寡糖，这将极大地有助于研究它们在 HS 中的天然作用。在这项研究中，通过对完全硫酸化的 dp2、dp4 和 dp6 进行化学修饰，制备了肝素寡糖文库，包括 dp2、dp4 和 dp6。优化了化学反应条件，以生成不同模式的 2-O-硫酸化和 6-O-硫酸化寡糖，然后通过高效液相色谱-离子阱/飞行时间质谱 (LC-IT-TOF/MS) 分析检测文库的二糖组成和结构。这为进一步研究 HS 中 O-硫酸化基团的结构和功能提供了基础。
