Department of Pathology, Yeungnam University College of Medicine, Gyeongsan, Republic of Korea.
Department of Urology, Yeungnam University College of Medicine, Gyeongsan, Republic of Korea.
In Vivo. 2021 Jan-Feb;35(1):299-306. doi: 10.21873/invivo.12259.
Minichromosome maintenance (MCM) proteins are involved in initiation of DNA replication and cell-cycle progression. Loss of MCM function results in genomic instability and causes carcinogenesis. Among MCM genes, the role and prognostic value of MCM6 expression in clear-cell renal cell carcinoma (ccRCC) has not been elucidated.
We assessed the mRNA expression level of MCM6 using the Gene Expression Profiling Interactive Analysis database and investigated MCM6 protein expression by immunohistochemistry in 238 ccRCC cases.
High MCM6 expression was significantly associated with increasing tumor size, pT, stage, tumor necrosis, and metastasis. Furthermore, high MCM6 expression was significantly associated with shorter overall and disease-free survival, and was an independent unfavorable prognostic marker. Regarding patients with metastasis, high MCM6-expressing ccRCC conferred significantly shorter survival than for those with low expression.
A high MCM6 expression level may be a promising biomarker to predict tumor progression, metastasis, and survival in patients with ccRCC.
微小染色体维持（MCM）蛋白参与 DNA 复制的起始和细胞周期进程。MCM 功能的丧失会导致基因组不稳定并引发致癌作用。在 MCM 基因中，MCM6 表达在透明细胞肾细胞癌（ccRCC）中的作用和预后价值尚未阐明。
我们使用基因表达谱交互分析数据库评估了 MCM6 的 mRNA 表达水平，并通过免疫组织化学方法在 238 例 ccRCC 病例中检测了 MCM6 蛋白的表达。
高 MCM6 表达与肿瘤大小增加、pT、分期、肿瘤坏死和转移显著相关。此外，高 MCM6 表达与总生存期和无病生存期缩短显著相关，是独立的不利预后标志物。对于发生转移的患者，高 MCM6 表达的 ccRCC 患者的生存时间明显短于低表达的患者。
MCM6 高表达水平可能是预测 ccRCC 患者肿瘤进展、转移和生存的有前途的生物标志物。
