Li Yukun, Zou Juan, Zhang Qunfeng, Quan Feifei, Cao Lu, Zhang Xiaodi, Liu Jue, Wu Daichao
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of University of South China, Hengyang, China.
Department of Obstetrics and Gynecology, Foshan First People's Hospital, Foshan, China.
Front Oncol. 2021 Jun 9;11:681261. doi: 10.3389/fonc.2021.681261. eCollection 2021.
Microliposome maintenance (MCM) 2, MCM3, MCM4, MCM5, MCM6, and MCM7 are DNA replication regulators and are involved in the progression of multiple cancer types, but their role in ovarian cancer is still unclear. The purpose of this study is to clarify the biological function and prognostic value of the MCM complex in ovarian cancer (OS) progression. We analyzed DNA alterations, mRNA and protein levels, protein structure, PPI network, functional enrichment, and prognostic value in OC based on the Oncomine, cBioPortal, TCGA, CPTAC, PDB, GeneMANIA, DAVID, KEGG, and GSCALite databases. The results indicated that the protein levels of these DNA replication regulators were increased significantly. Moreover, survival analysis showed a prognostic signature based on the MCM complex, which performed moderately well in terms of OS prognostic prediction. Additionally, protein structure, functional enrichment, and PPI network analyses indicated that the MCM complex synergistically promoted OC progression by accelerating DNA replication and the cell cycle. In conclusion, our study suggested that the MCM complex might be a potential target and prognostic marker for OC patients.
微小染色体维持蛋白（MCM）2、MCM3、MCM4、MCM5、MCM6和MCM7是DNA复制调节因子，参与多种癌症类型的进展，但其在卵巢癌中的作用仍不清楚。本研究的目的是阐明MCM复合体在卵巢癌（OC）进展中的生物学功能和预后价值。我们基于Oncomine、cBioPortal、TCGA、CPTAC、PDB、GeneMANIA、DAVID、KEGG和GSCALite数据库分析了OC中的DNA改变、mRNA和蛋白质水平、蛋白质结构、蛋白质-蛋白质相互作用（PPI）网络、功能富集和预后价值。结果表明，这些DNA复制调节因子的蛋白质水平显著升高。此外，生存分析显示基于MCM复合体的预后特征，其在OS预后预测方面表现中等。此外，蛋白质结构、功能富集和PPI网络分析表明，MCM复合体通过加速DNA复制和细胞周期协同促进OC进展。总之，我们的研究表明，MCM复合体可能是OC患者的潜在靶点和预后标志物。
