古蔺内生真菌的分离培养、鉴定及其抗真菌活性
Culture Separation, Identification and Unique Anti-pathogenic Fungi Capacity of Endophytic Fungi from Gucheng .
机构信息
School of Pharmacy, Anhui University of Chinese Medicine, Hefei, P.R. China.
Anhui Province Key Laboratory of Research and Development of Chinese Medicine, Hefei, P.R. China.
出版信息
In Vivo. 2021 Jan-Feb;35(1):325-332. doi: 10.21873/invivo.12263.
BACKGROUND
Salvia miltiorrhiza is a medical herb for human disorders including cardiovascular diseases and cancer. However, the interactions between Salvia miltiorrhiza and its endophytes are largely unknown. The current study aimed at identifying its endophytic fungi and examining their inhibitory effects on anti-pathogenic fungus.
MATERIALS AND METHODS
Distinct species of endophytic fungi were isolated from the roots of Salvia miltiorrhiza, cultured, sequenced, aiming to predict their taxonomical structures. Meanwhile, extracts from each endophytic fungus fermentations were isolated, compared and evaluated on the inhibitory efficacies on five pathological fungi, Cercospora nicotianae, Phoma arachnidicola, Staphylococcus, Phytophthora eggplant, and Rhizoctonia cerealis.
RESULTS
A total of 34 strains of endophytic fungi were obtained from Salvia miltiorrhiza. Among them, SX19 and C. Gloeosporioids exhibited the most effective inhibitions on five pathogenic fungi.
CONCLUSION
The anti-fungal activities of the endophytic fungus from Salvia miltiorrhiza were confirmed for the first time, and this may benefit crop quality and production in the future.
背景
丹参是一种用于治疗人类疾病的草药，包括心血管疾病和癌症。然而，丹参与其内生真菌之间的相互作用在很大程度上是未知的。本研究旨在鉴定其内生真菌，并研究其对抗病原真菌的抑制作用。
材料与方法
从丹参根部分离出不同种类的内生真菌，进行培养、测序，以预测其分类结构。同时，从每个内生真菌发酵物中分离、比较和评估其对五种病原菌（烟草尾孢菌、茄丝核菌、葡萄球菌、茄腐霉和立枯丝核菌）的抑制效果。
结果
从丹参中获得了 34 株内生真菌。其中，SX19 和 C. Gloeosporioids 对五种病原菌的抑制作用最强。
结论
首次证实了丹参内生真菌的抗真菌活性，这可能有助于未来提高作物质量和产量。
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