突触前M1毒蕈碱受体调节大鼠基底前脑切片中乙酰胆碱的自发释放。
Presynaptic M1 muscarinic receptor modulates spontaneous release of acetylcholine from rat basal forebrain slices.
作者信息
Suzuki T, Fujimoto K, Oohata H, Kawashima K
机构信息
Department of Pharmacology, Kyoritsu College of Pharmacy, Tokyo, Japan.
出版信息
Neurosci Lett. 1988 Jan 22;84(2):209-12. doi: 10.1016/0304-3940(88)90409-0.
Spontaneous release of acetylcholine (ACh) from rat basal forebrain slices in the presence of cholinesterase inhibitor was directly determined using a specific radioimmunoassay for ACh. The release was calcium dependent. A consistent amount of ACh release was observed throughout the experiment. Atropine (10(-8) to 10(-5) M) and pirenzepine (10(-7) to 10(-5) M) enhanced spontaneous ACh release. These findings indicate the presence of an M1 muscarinic autoreceptor that modulates spontaneous release of ACh in the rat basal forebrain.
在存在胆碱酯酶抑制剂的情况下，使用针对乙酰胆碱（ACh）的特异性放射免疫测定法直接测定大鼠基底前脑切片中乙酰胆碱的自发释放。这种释放是钙依赖性的。在整个实验过程中观察到一致量的乙酰胆碱释放。阿托品（10^(-8)至10^(-5) M）和哌仑西平（10^(-7)至10^(-5) M）增强了乙酰胆碱的自发释放。这些发现表明存在一种M1毒蕈碱型自身受体，它调节大鼠基底前脑中乙酰胆碱的自发释放。
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