Murawski M, Schwarz T, Erak V, Sohal J, Ahmadi B, Kridli R, Bartlewski P
Department of Nutrition, Animal Biotechnology and Fisheries, Faculty of Animal Science, University of Agriculture in Kraków, Cracow, Poland.
Department of Genetics, Animal Breeding and Ethology, Faculty of Animal Science, University of Agriculture in Kraków, Cracow, Poland.
Arch Razi Inst. 2021 Jan;75(4):484-490. doi: 10.22092/ari.2020.351712.1530. Epub 2021 Jan 1.
The specific changes in antral follicle numbers and wave-like development have remained unrevealed in cyclic ewes fed high-protein, high-energy lupin grain for 6 days during the luteal phase of the estrous cycle (i.e., short-term nutritional flushing). This study was mainly conducted to determine ovarian effects of the 6-day lupin grain feeding in non-prolific Polish Mountain ewes, using transrectal ovarian ultrasonography and abdominal videoendoscopy. Estrus and ovulations were synchronized in 24 ewes with progestin-releasing intravaginal sponges for 12 days during the middle portion of the breeding season (September-October; 50.0458°N, 19.8406°E). Twenty-four ewes were assigned to three equal groups (n=8 each), including the Control group being fed the maintenance diet (i.e., hay-only), Treatment 1 receiving 500 g of lupin grain once a day, and Treatment 2 receiving 250 g of lupin grain twice a day, from days 9-14 of the synchronized estrous cycle (day 0=first ovulation of the interovulatory period studied). No differences were observed in the mean ovulation rate among the three groups of Polish Mountain ewes (P>0.05). Ovarian antral follicles emerging in the penultimate wave of the estrous cycle in Treatment 2 ewes had a longer growth phase (p <0.05) and attained a greater diameter (p <0.05) before ovulation, in comparison to those in the other two groups. A final wave of the interovulatory interval emerged ~1 day earlier in Treatment 2 than in Treatment 1 ewes (p <0.05). Nutritional supplementation with lupin grain increased the number of 3-mm follicles in Treatment 2 ewes (p <0.05). The results of this study indicated that short-term nutritional flushing with lupin grain from mid- to late luteal phase did not consistently enhance ovulatory responses in non-prolific genotypes of ewes. Although the administration of lupins altered the timing of wave emergence, ovulatory follicle diameter, or duration of different stages of the follicular lifespan, it failed to increase the number of ovulatory follicles emerging in the penultimate and final waves of the estrous cycle in non-prolific Polish Mountain sheep.
在发情周期的黄体期给周期性发情的母羊饲喂6天高蛋白、高能量的羽扇豆谷物（即短期营养冲洗）后，窦卵泡数量和波状发育的具体变化仍未明确。本研究主要通过经直肠卵巢超声检查和腹部视频内窥镜检查，来确定在非多产的波兰山地母羊中，6天羽扇豆谷物饲喂对卵巢的影响。在繁殖季节中期（9月至10月；北纬50.0458°，东经19.8406°），用释放孕激素的阴道海绵栓使24只母羊的发情和排卵同步12天。将24只母羊平均分为三组（每组n = 8只），包括对照组，饲喂维持日粮（即仅喂干草）；处理1组，从同步发情周期的第9 - 14天（第0天=所研究的卵泡期的第一次排卵）开始，每天喂500克羽扇豆谷物；处理2组，每天喂2次，每次250克羽扇豆谷物。在三组波兰山地母羊中，平均排卵率未观察到差异（P>0.05）。与其他两组相比，处理2组母羊在发情周期倒数第二个波中出现的卵巢窦卵泡在排卵前具有更长的生长期（p <0.05）和更大的直径（p <0.05）。处理2组卵泡期的最后一个波比处理1组母羊提前约1天出现（p <0.05）。用羽扇豆谷物进行营养补充增加了处理2组母羊中3毫米卵泡的数量（p <0.05）。本研究结果表明，在黄体期中期至晚期用羽扇豆谷物进行短期营养冲洗并不能持续增强非多产基因型母羊的排卵反应。虽然羽扇豆的施用改变了波出现的时间、排卵卵泡直径或卵泡寿命不同阶段的持续时间，但它未能增加非多产波兰山地羊发情周期倒数第二个波和最后一个波中出现的排卵卵泡数量。
