Somchit A, Campbell B K, Khalid M, Kendall N R, Scaramuzzi R J
Department of Veterinary Basic Sciences, Royal Veterinary College, North Mimms, Hertfordshire, UK.
Theriogenology. 2007 Oct 15;68(7):1037-46. doi: 10.1016/j.theriogenology.2007.08.001. Epub 2007 Sep 10.
An experiment was conducted using 16 cyclic, Welsh Mountain ewes during the luteal phase of the estrous cycle to determine the effect of a 5-day period of feeding a high-energy high-protein diet (lupin grain; 500 g/day) on folliculogenesis and on the plasma concentrations of glucose, insulin, follicle stimulating hormone (FSH) and estradiol-17beta, and on the follicular fluid concentrations of glucose, inhibin A, estradiol-17beta, androstenedione and progesterone. Average weight did not differ between lupin-fed and control groups during the experiment. There was a trend for the number of small and large follicles to increase in the lupin-fed group. The plasma concentrations of glucose (P=0.012) and insulin (P=0.007) were higher during the feeding period in lupin-fed ewes. The plasma concentrations of FSH and estradiol-17beta were not significantly different. The mean follicular fluid concentration of glucose (small follicles; <3.5 mm) from lupin-fed ewes was elevated (P=0.010) and progesterone lowered (P=0.034) compared to controls. The follicular fluid concentrations of estradiol-17beta, androstenedione and inhibin A were not significantly different. The follicular fluid concentration of estradiol-17beta was positively correlated with androstenedione (r=-0.241; P=0.001) and inhibin A (r=0.734; P< or =0.001) and glucose was negatively correlated with inhibin (r=-0.241; P=0.01), but not estradiol (r=0.075; P=0.410) or androstenedione (r=0.050; P=0.564). The lupin grain supplement increased the number of follicles as expected, but this increase was not significant. These changes were reflected in follicular fluid where lupin feeding increased the concentration of glucose and decreased the concentration of progesterone in follicles less than 3.5mm in diameter. These data suggest that the local ovarian actions of nutrients have a role in the mediation of nutritional influences on folliculogenesis.
选用16只处于发情周期黄体期的威尔士山地母羊进行实验，以确定连续5天饲喂高能高蛋白日粮（羽扇豆籽实；500克/天）对卵泡发生、血浆葡萄糖、胰岛素、促卵泡激素（FSH）和雌二醇-17β浓度以及卵泡液葡萄糖、抑制素A、雌二醇-17β、雄烯二酮和孕酮浓度的影响。实验期间，羽扇豆饲喂组和对照组母羊的平均体重无差异。羽扇豆饲喂组中小卵泡和大卵泡数量有增加趋势。羽扇豆饲喂母羊在饲喂期血浆葡萄糖浓度（P = 0.012）和胰岛素浓度（P = 0.007）较高。血浆FSH和雌二醇-17β浓度无显著差异。与对照组相比，羽扇豆饲喂母羊卵泡液葡萄糖平均浓度（小卵泡；<3.5毫米）升高（P = 0.010），孕酮浓度降低（P = 0.034）。卵泡液中雌二醇-17β、雄烯二酮和抑制素A浓度无显著差异。卵泡液中雌二醇-17β浓度与雄烯二酮呈正相关（r = -0.241；P = 0.001），与抑制素A呈正相关（r = 0.734；P≤0.001），葡萄糖与抑制素呈负相关（r = -0.241；P = 0.01），但与雌二醇（r = 0.075；P = 0.410）或雄烯二酮（r = 0.050；P = 0.564）无相关性。如预期的那样，羽扇豆籽实补充料增加了卵泡数量，但这种增加不显著。这些变化反映在卵泡液中，羽扇豆饲喂增加了直径小于3.5毫米卵泡中葡萄糖的浓度，降低了孕酮的浓度。这些数据表明，营养物质在卵巢局部的作用在介导营养对卵泡发生的影响中发挥作用。
