Plant Pathology and Plant-Microbe Biology Section, School of Integrative Plant Science, Cornell University, Ithaca, NY 14853, U.S.A.
Emerging Pests and Pathogens Research Unit, Robert W. Holley Center, U.S. Department of Agriculture Agricultural Research Service, Ithaca, NY 14853, U.S.A.
Plant Dis. 2021 Sep;105(9):2585-2594. doi: 10.1094/PDIS-09-20-2089-RE. Epub 2021 Oct 24.
Soft rot bacteria classified in the Pectobacteriaceae (SRP), including and spp., are responsible for soft rot and blackleg diseases of potato. Since 2014, blackleg outbreaks caused by have increased in the United States and Canada. Our previous study found that the most abundant causal organisms of blackleg disease in New York State were and , with the latter being the only species reported. In the present study, we identified and characterized pathogenic SRP bacteria from 19 potato samples collected in New York State during the 2017 growing season. We used genome sequence comparison to determine the pathogens' species. We found eight , one , two , two , and six isolates in our 2017 SRP collection. This is the first time that has been reported to cause potato blackleg disease in New York State. We determined the phylogenetic relationships between the SRP strains by using 151 single-copy orthologous gene sequences shared among the set of bacteria in our analysis, which provided better resolution than phylogenies constructed with the gene.
软腐细菌分类在果胶杆菌科（SRP），包括 和 spp.，是造成马铃薯软腐病和黑胫病的原因。自 2014 年以来，美国和加拿大的 引起的黑胫病爆发有所增加。我们之前的研究发现，在纽约州黑胫病的最主要病原体是 和 ，后者是唯一报告的 种。在本研究中，我们从 2017 年生长季节在纽约州采集的 19 个马铃薯样本中鉴定和特征化了致病性 SRP 细菌。我们使用基因组序列比较来确定病原体的种类。我们在 2017 年的 SRP 分离物中发现了八个 、一个 、两个 、两个 和六个 分离物。这是首次报道 在纽约州引起马铃薯黑胫病。我们通过使用在我们的分析中共享的 151 个单拷贝直系同源基因序列来确定 SRP 菌株之间的系统发育关系，这比使用 基因构建的系统发育提供了更好的分辨率。
