State Key Laboratory for Agrobiotechnology/Key Laboratory of Crop Heterosis and Utilization, the Ministry of Education/Key Laboratory of Crop Genetic Improvement, Beijing Municipality/China Agricultural University, Beijing, 100193, China.
National Plant Gene Research Centre, Beijing, 100193, China.
Theor Appl Genet. 2021 Mar;134(3):835-847. doi: 10.1007/s00122-020-03734-6. Epub 2021 Jan 6.
A novel wax locus GLOSSY1 was finely mapped to an approximately 308.1-kbp genomic interval on chromosome 2DS of wheat. The epicuticular wax, the outermost layer of aerial organs, gives plants their bluish-white (glaucous) appearance. Epicuticular wax is ubiquitous and provides an essential protective function against environmental stresses. In this study, we identified the glossy1 mutant on the basis of its glossy glume from an EMS population in the elite wheat (Triticum aestivum L.) cultivar Jimai22. The mutant had a dramatically different profile in total wax load and composition of individual wax constituents relative to the wild type, resulting in the increased cuticle permeability of glumes. The glossy glume phenotype was controlled by a single, semidominant locus mapping to the short arm of chromosome 2D, within a 308.1-kbp genomic interval that contained ten annotated protein-coding genes. These results pave the way for an in-depth analysis of the underlying genetic basis of wax formation patterns and enrich our understanding of mechanisms regulating wax metabolism.
一个新的蜡质基因 GLOSSY1 被精细地定位到小麦 2DS 染色体上大约 308.1-kbp 的基因组区间内。表皮蜡质是气生器官的最外层，赋予植物淡蓝色（苍白）的外观。表皮蜡质无处不在，为植物提供了重要的保护功能，抵御环境压力。在这项研究中，我们根据 EMS 群体中来自优质小麦（Triticum aestivum L.）品种金麦 22 的蜡质颖壳，鉴定出 glossy1 突变体。与野生型相比，突变体的总蜡载量和个别蜡质成分的组成有明显不同，导致颖壳的角质层通透性增加。光滑颖壳表型由一个单基因控制，该基因位于 2D 染色体的短臂上，位于包含十个注释蛋白编码基因的 308.1-kbp 基因组区间内。这些结果为深入分析蜡质形成模式的遗传基础铺平了道路，并丰富了我们对调节蜡质代谢机制的理解。
