School of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Lianhua Road 100#, Zhengzhou High & New Technology Industries Development Zone, Zhengzhou, 450001, Henan Province, People's Republic of China.
Mikrochim Acta. 2021 Jan 6;188(1):23. doi: 10.1007/s00604-020-04666-0.
A "signal off" aptasensor has been developed to detect deoxynivalenol (DON). DON aptamers (Apt) were used as biological recognition elements, nickel ferrite nanotubes (NiFeO NTs) are used as the base material to increase the surface area of the electrode, and the Au@Pt NRs were used as carriers for loading signal labels thionine (Thi) and complementary strand (cDNA). In the presence of DON it will be specifically captured by Apt, then the competition mechanism was triggered; the signal molecules fall off from the electrode surface, which then causes the electrode signal to decrease. NiFeO NTs and Au@Pt NRs were characterized by transmission electron microscope (TEM), scanning electron micrograph (SEM), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD). The designed sensor provides a concentration range of 1 × 10 to 5 × 10 mg mL and limit of detection of 3.02 × 10 mg mL. Determination of DON in corn meal samples was investigated and the recovery was 98.4 to 103.5%. The proposed aptasensor displayed good sensitivity, high specificity, and acceptable reproducibility. Graphical abstract Based on NiFeO NTs as substrate material and Au@Pt NRs as signal label prepared DON aptasensor for the determination of DON.
已开发出一种“信号关闭”适体传感器来检测脱氧雪腐镰刀菌烯醇 (DON)。DON 适体 (Apt) 用作生物识别元件，镍铁氧体纳米管 (NiFeO NTs) 用作基底材料以增加电极的表面积，并且 Au@Pt NRs 用作负载信号标签硫堇 (Thi) 和互补链 (cDNA) 的载体。在 DON 的存在下，它将被 Apt 特异性捕获，然后触发竞争机制；信号分子从电极表面脱落，这导致电极信号降低。NiFeO NTs 和 Au@Pt NRs 通过透射电子显微镜 (TEM)、扫描电子显微镜 (SEM)、能量色散 X 射线光谱 (EDS) 和 X 射线衍射 (XRD) 进行了表征。所设计的传感器提供了 1×10 至 5×10 mg mL 的浓度范围和 3.02×10 mg mL 的检测限。对玉米粉样品中的 DON 进行了测定，回收率为 98.4%至 103.5%。该适体传感器表现出良好的灵敏度、高特异性和可接受的重现性。
