National Institute of Oceanography and Fishers, Alexandria, Egypt.
Pharmacognosy Department, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
Environ Sci Pollut Res Int. 2021 Apr;28(16):19664-19676. doi: 10.1007/s11356-020-11892-9. Epub 2021 Jan 6.
Brown seaweeds are rich in polysaccharides, such as fucoidan (FUC) which has shown beneficial effects in several medical conditions. The aim of the present study was to assess the antioxidant, anti-inflammatory, and hepatoprotective properties of Colpomenia sinuosa- and Sargassum prismaticum-isolated FUC in vitro and in vivo. The hot acid extraction method was used to isolate FUC from C. sinuosa (FCS) and S. prismaticum (FSP) species. The antioxidant, anticancer, as well as the effect on neurotransmitter-degrading enzyme and disaccharidase activities were measured using standard protocols. Moreover, the hepatoprotective effect of two FCS doses (100 and 200 mg/kg) on paracetamol-administered rats (one dose of 1 g/kg) were evaluated by measuring blood liver function markers, hepatic pro-oxidants as malondialdehyde (MDA) and nitric oxide (NO), antioxidants as glutathione (GSH) and glutathione peroxidase (GPx), proinflammatory markers as inducible nitric oxide synthase (iNOS), interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α), and liver histology. The crude fucoidan yield was 15.6% and 14.8% of C. sinuosa and S. prismaticum dry weights, respectively. The antioxidant effects and cytotoxic activity on hepatic cancer cell were higher for FCS than FSP. Moreover, in vivo data showed that FCS administration at both doses significantly improved liver functions and alleviated histological alterations, hepatic inflammation, and oxidative stress following paracetamol intake. In conclusion, fucoidan exerts anti-inflammatory, antidigestive enzyme activity, antioxidant, anticancer, and hepatoprotective effects.
褐藻富含多糖，如岩藻聚糖硫酸酯 (FUC)，它在多种医学病症中显示出有益的作用。本研究的目的是评估 Colpomenia sinuosa 和 Sargassum prismaticum 中分离的 FUC 的体外和体内抗氧化、抗炎和保肝特性。采用热酸提取法从 C. sinuosa（FCS）和 S. prismaticum（FSP）中分离 FUC。使用标准方案测量抗氧化剂、抗癌以及对神经递质降解酶和二糖酶活性的影响。此外，通过测量血液肝功能标志物、肝过氧化物如丙二醛 (MDA) 和一氧化氮 (NO)、抗氧化剂如谷胱甘肽 (GSH) 和谷胱甘肽过氧化物酶 (GPx)、诱导型一氧化氮合酶 (iNOS)、白细胞介素 1β (IL-1β)、肿瘤坏死因子-α (TNF-α) 和肝组织学来评估两种 FCS 剂量（100 和 200 mg/kg）对乙酰氨基酚给药大鼠（一剂 1 g/kg）的保肝作用。褐藻粗聚糖的得率分别为 C. sinuosa 和 S. prismaticum 干重的 15.6%和 14.8%。FCS 的抗氧化作用和对肝癌细胞的细胞毒性活性均高于 FSP。此外，体内数据表明，FCS 在两个剂量下给药均可显著改善肝功能，减轻组织学改变、肝炎症和氧化应激，同时摄入对乙酰氨基酚。总之，褐藻聚糖硫酸酯具有抗炎、抗消化酶活性、抗氧化、抗癌和保肝作用。
