Solaymani Shahram, Nezafat Negin Beryani, Ţălu Ştefan, Shafiekhani Azizollah, Dalouji Vali, Amiri Ali, Rezaee Sahar, Morozov Ilya A
School of Physics, Institute for Research in Fundamental Sciences, Tehran, Iran.
Department of Physics, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran.
Microsc Res Tech. 2021 Jun;84(6):1098-1105. doi: 10.1002/jemt.23668. Epub 2021 Jan 6.
The main goal of the present work is to explore the three dimensional (3-D) atomic force microscopy (AFM) images of human teeth and investigating their micromorphology. For this purpose, 10 fresh and permanent canine teeth were selected from a group of 40-year-old men who were candidate for the experimental processes. Afterward, they were all applied for studying the morphology of their hard tissues. The tapping mode of AFM was used to characterize the surface micromorphology on the square areas of 1 μm × 1 μm (512 × 512 pts). AFM results and surface stereometric analysis indicate the relationships between the micromorphology of the surface and the structural properties of these tissues across the length scales. As can be seen, the surface of cementum has the most irregular topography (D = 2.87 ± 0.01) while the most regular topography (D = 2.43 ± 0.01) is found in dentin. Furthermore, the more and less regularity of the surface have been found in inner enamel (Sq = 26.26 nm) and dentin (Sq = 41.28 nm), respectively. Stereometric and fractal analyses give valuable information about human canine teeth via 3-D micromorphology.
本研究的主要目标是探索人类牙齿的三维（3-D）原子力显微镜（AFM）图像，并研究其微观形态。为此，从一组40岁男性中选取了10颗新鲜的恒牙犬齿，这些男性是实验过程的候选对象。之后，将它们全部用于研究其硬组织的形态。采用AFM的轻敲模式来表征1μm×1μm（512×512点）方形区域的表面微观形态。AFM结果和表面立体测量分析表明了表面微观形态与这些组织在不同长度尺度上的结构特性之间的关系。可以看出，牙骨质表面的地形最不规则（D = 2.87±0.01），而在牙本质中发现了最规则的地形（D = 2.43±0.01）。此外，在内釉质（Sq = 26.26nm）和牙本质（Sq = 41.28nm）中分别发现了表面规则性的增加和减少。立体测量和分形分析通过3-D微观形态提供了有关人类犬齿的有价值信息。
