Nakatsuji Hirotaka, Matsusaki Michiya
Joint Research Laboratory (TOPPAN) for Advanced Cell Regulatory Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Department of Applied Chemistry, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
ACS Biomater Sci Eng. 2019 Nov 11;5(11):5610-5614. doi: 10.1021/acsbiomaterials.9b00090. Epub 2019 May 3.
In vitro construction of highly organized three-dimentional (3D) tissues is still a key challenge for tissue engineering. In this study, we fabricated multilayered tissues composed of extracellular matrix (ECM) layer and cell layer by stacking cell-seeded ECM papers. A paperlike scaffold was prepared by simply casting dispersion of microfibered ECM. We showed the paperlike scaffold is a superior material for constructing 3D tissue because of its high permeability and cell migration ability, and our method can control the thickness and component of ECM in multilayered 3D tissues. It can contribute to construction of normal and disease tissue models.
体外构建高度有序的三维（3D）组织仍然是组织工程面临的关键挑战。在本研究中，我们通过堆叠接种细胞的细胞外基质（ECM）纸来制造由ECM层和细胞层组成的多层组织。通过简单地浇铸微纤维ECM分散液制备了一种纸状支架。我们表明，这种纸状支架因其高渗透性和细胞迁移能力，是构建3D组织的优质材料，并且我们的方法可以控制多层3D组织中ECM的厚度和成分。它有助于构建正常和疾病组织模型。
