Son Jaejung, Bang Min Seo, Park Je-Kyun
Department of Bio and Brain Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
ACS Biomater Sci Eng. 2019 Jan 14;5(1):339-345. doi: 10.1021/acsbiomaterials.8b01066. Epub 2018 Dec 4.
Modular tissue engineering creates a three-dimensional (3D) macroscale tissue construct from modular microscale units for complex 3D tissue reconstruction. In particular, a hydrogel sheet that is one of the module types allows easy and controllable assembly of 3D microenvironments as compared to other module types such as a microcapsule and a microfiber. However, it is difficult to manipulate a hydrogel sheet made of extracellular matrix (ECM) proteins. Thus, in this study, we propose a fabrication technique for the manipulation of thin collagen sheet containing cells with a paper support. A donut-shaped paper support was combined with the micropatterned collagen sheet by permeation of cell-collagen mixture passing through a micropatterned mold into the paper. We established a sterile method for the paper support to maintain high cell viability and intrinsic cell morphology and demonstrated an endothelial module having 3D tubular networks. The endothelial module also showed that control of the pattern length resulted in a change in the tubular size and network density within a sheet. The geometrically controlled collagen sheet modules are expected to be used for improved implantation and biologically relevant drug testing.
模块化组织工程通过模块化的微观单元构建三维（3D）宏观组织构建体，用于复杂的3D组织重建。特别是，水凝胶片作为模块类型之一，与微胶囊和微纤维等其他模块类型相比，能实现3D微环境的轻松且可控组装。然而，由细胞外基质（ECM）蛋白制成的水凝胶片难以操控。因此，在本研究中，我们提出一种利用纸质支撑物操控含细胞薄胶原片的制造技术。通过使细胞 - 胶原混合物穿过微图案模具渗透到纸中，将甜甜圈形状的纸质支撑物与微图案化的胶原片结合。我们建立了一种用于纸质支撑物的无菌方法，以维持高细胞活力和细胞固有形态，并展示了具有3D管状网络的内皮模块。内皮模块还表明，图案长度的控制导致片内管状尺寸和网络密度的变化。几何形状受控的胶原片模块有望用于改善植入和进行生物学相关的药物测试。
