半胱氨酸水平的降低会导致蚊子衰老过程中的谷胱甘肽缺乏。

A decrease in cysteine levels causes the glutathione deficiency of aging in the mosquito.

作者信息

Richie J P, Lang C A

机构信息

Department of Biochemistry, University of Louisville School of Medicine, Kentucky 40292.

出版信息

Proc Soc Exp Biol Med. 1988 Feb;187(2):235-40. doi: 10.3181/00379727-187-42660.

DOI:10.3181/00379727-187-42660

PMID:3340632

Abstract

Our previous results indicated that a glutathione (GSH) deficiency is a determinant of the aging process in many tissues and organisms. Correction of this deficiency in the aging mosquito by feeding the cysteine (Cys) precursor magnesium thiazolidine carboxylic acid (MgTc) suggested that the cause could be a lack of Cys. Adult mosquitoes (Aedes aegypti) were fed either a control diet or a diet supplemented with MgTC and then were analyzed for their Cys, cystine, GSH, and glutathione disulfide contents with our HPLC method. The life span profile of Cys levels paralleled that of GSH in the control group with high levels in the young that decreased during maturity and aging. Cystine and glutathione disulfide were undetectable. The causal relationship between the Cys and the GSH deficiencies was shown in the MgTC-supplemented group with an 83% increase in Cys and a 39% increase in GSH relative to control values. Further the conversion steps of MgTC to Cys and then to GSH were verified by use of buthionine sulfoximine. These results demonstrate that a Cys deficiency occurs in the aging mosquito and is the cause of the GSH deficiency.

摘要

我们之前的研究结果表明，谷胱甘肽（GSH）缺乏是许多组织和生物体衰老过程的一个决定因素。通过给衰老的蚊子喂食半胱氨酸（Cys）前体噻唑烷羧酸镁（MgTc）来纠正这种缺乏，这表明原因可能是缺乏Cys。给成年蚊子（埃及伊蚊）喂食对照饮食或添加了MgTC的饮食，然后用我们的高效液相色谱法分析它们的Cys、胱氨酸、GSH和谷胱甘肽二硫化物含量。在对照组中，Cys水平的寿命分布与GSH的寿命分布相似，在年轻个体中含量较高，在成熟和衰老过程中下降。未检测到胱氨酸和谷胱甘肽二硫化物。在添加MgTC的组中显示了Cys缺乏与GSH缺乏之间的因果关系，相对于对照值，Cys增加了83%，GSH增加了39%。此外，通过使用丁硫氨酸亚砜胺验证了MgTC向Cys然后向GSH的转化步骤。这些结果表明，衰老的蚊子中存在Cys缺乏，这是GSH缺乏的原因。