血清反应因子，急性肾损伤的新型早期诊断生物标志物。
Serum response factor, a novel early diagnostic biomarker of acute kidney injury.
机构信息
Department of Nephrology, The Affiliated Hospital of Qingdao University, Qingdao 266003, China.
Department of Obstetrics, Weifang People's Hospital, Weifang 261041, China.
出版信息
Aging (Albany NY). 2021 Jan 5;13(2):2885-2894. doi: 10.18632/aging.202381.
OBJECTIVE
Studies have shown that serum response factor (SRF) is increased in chronic kidney injury, such as diabetic nephropathy, hyperuricemic nephropathy and renal cell carcinoma. The objective is to explore the early diagnostic value of SRF in acute kidney injury (AKI).
METHODS
AKI-related microarray data were analyzed, and the expression and location of SRF were investigated in the early phase of AKI.
RESULTS
Bioinformatics results demonstrated that SRF was dramatically elevated 2-4 h after ischemia/reperfusion (I/R) in mouse renal tissue. In I/R rats, SRF was mostly expressed and located in renal tubular epithelial cells (TECs). SRF started to increase at 1 h, peaked at 3-9 h and started to decrease at 12 h after I/R. The areas under the ROC curve of renal SRF mRNA, renal SRF protein, urinary SRF, serum SRF and serum creatinine (Scr) were 87.9%, 83.0%, 81.3%, 78.8%, 68.8%, respectively.
CONCLUSION
SRF is remarkably upregulated in early (before 24 h) AKI and can replace Scr as a potential new early diagnostic biomarker of AKI.
目的
研究表明，血清反应因子（SRF）在慢性肾损伤中增加，如糖尿病肾病、高尿酸血症肾病和肾细胞癌。本研究旨在探讨 SRF 在急性肾损伤（AKI）中的早期诊断价值。
方法
分析 AKI 相关的微阵列数据，并研究 SRF 在 AKI 早期的表达和定位。
结果
生物信息学结果表明，在小鼠肾组织缺血/再灌注（I/R）后 2-4 小时，SRF 显著升高。在 I/R 大鼠中，SRF 主要在肾小管上皮细胞（TECs）中表达和定位。SRF 在 I/R 后 1 小时开始增加，在 3-9 小时达到峰值，并在 12 小时开始下降。肾 SRF mRNA、肾 SRF 蛋白、尿 SRF、血清 SRF 和血清肌酐（Scr）的 ROC 曲线下面积分别为 87.9%、83.0%、81.3%、78.8%和 68.8%。
结论
SRF 在早期（24 小时内）AKI 中显著上调，可替代 Scr 作为 AKI 的潜在新的早期诊断生物标志物。
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