Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, China; College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang, China.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang, China; College of Marine Life and Fisheries, Jiangsu Ocean University, Lianyungang, China.
Food Chem. 2021 May 1;343:128393. doi: 10.1016/j.foodchem.2020.128393. Epub 2020 Oct 16.
The denaturation temperature of collagen has been determined using several methods, such as circular dichroism, fourier transform infrared spectroscopy, and differential scanning calorimetry, etc. Such methods need specific equipment or mass samples (more than 75 mg samples), which means higher inspection costs. In this study, Sirius red was employed to determine the Tds of collagen from calf tendon, silver carp skin, frog and salmon skins (38.2 °C, 32.6 °C, 33.8 °C, and 15.6 °C, respectively) to explore a new method that does not require special equipment and only needs 2-3 mg sample for one measurement. This method was suitable for the determination of the denaturation temperature of collagen from terrestrial, aquatic and amphibian animals. Analysis of variance and t-test revealed that no significant difference was found between Sirius Red and viscosity methods. However, the Sirius Red method needs simpler equipment and less sample than viscosity and other methods. So it could be used as a convenient approach to determine the denaturation temperature of collagen instead of the viscosity method.
已有多种方法（如圆二色性、傅里叶变换红外光谱和差示扫描量热法等）用于测定胶原蛋白的变性温度。这些方法需要特定的设备或大量样品（超过 75mg 样品），这意味着检测成本更高。在本研究中，我们使用 Sirius 红测定了小牛跟腱、银鲤鱼皮、青蛙皮和三文鱼皮胶原蛋白的 Tds（分别为 38.2°C、32.6°C、33.8°C 和 15.6°C），以探索一种不需要特殊设备、仅需 2-3mg 样品即可进行一次测量的新方法。该方法适用于测定陆生、水生和两栖动物胶原蛋白的变性温度。方差分析和 t 检验显示，Sirius 红法与黏度法之间无显著性差异。然而，Sirius 红法所需设备比黏度法等其他方法更简单，样品用量也更少。因此，它可以作为一种方便的方法来测定胶原蛋白的变性温度，代替黏度法。
