Department of Plant Science, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Department of Genetics, Faculty of Agriculture, University of Tanta, Tanta 31111, Egypt.
Phytopathology. 2021 Aug;111(8):1410-1419. doi: 10.1094/PHYTO-10-20-0482-R. Epub 2021 Sep 22.
Reactive oxygen species (ROSs) represent one of the first lines of plants' biochemical defense against pathogens. Plants' respiratory burst oxidase homologs (RBOHs) produce ROSs as byproducts in several cellular compartments. In potato tubers, respiratory burst oxidase homolog (StRBOHs) are involved in suberization and healing of wounded tissues. StRbohA has been tested in the model plant , which led to enhanced plant defense against the soilborne pathogen . Here, we showed that overexpressing in potato plants increases plant tolerance to the oomycete , the causal agent of late blight disease. Transgenic potato plants expressing showed reduced disease symptoms (necrosis) compared with the wild type. In parallel, the expression of pathogenesis-related genes (); ; antioxidation-related genes , , , , and ; and genes involved in the biosynthesis pathways of jasmonic and salicylic acids (, , , , , and ) exhibited significant increases in transgenic plants in response to infection. After higher expression of , ROSs accumulated more in inoculation sites of the transgenic plants. ROSs act as signals that activate gene expression in the salicylic acid (SA) biosynthesis pathway, leading to the accumulation of SA and triggering SA-based defense mechanisms. SA-responsive showed higher expression in the transgenic plants, which resulted in the restriction of pathogen growth in plant tissues. These results demonstrate the effective role of StRbohA in increasing potato defense against .
活性氧 (ROS) 是植物对抗病原体的第一道生化防御线之一。植物的呼吸爆发氧化酶同源物 (RBOHs) 在几个细胞区室中产生 ROS 作为副产物。在土豆块茎中，呼吸爆发氧化酶同源物 (StRBOHs) 参与了创伤组织的木质化和愈合。StRbohA 在模式植物中进行了测试，这导致植物对土传病原体的防御能力增强。在这里，我们表明在土豆植物中过度表达 会增加植物对卵菌的耐受性，卵菌是晚疫病的病原体。与野生型相比，表达 的转基因土豆植物表现出减少的疾病症状（坏死）。平行地，与发病相关的基因的表达（）；；抗氧化相关基因、、、、和；以及参与茉莉酸和水杨酸生物合成途径的基因（、、、、、和）在转基因植物中对感染的反应显著增加。在 更高表达后，ROS 在转基因植物的接种部位积累更多。ROS 作为信号激活了水杨酸 (SA) 生物合成途径中的基因表达，导致 SA 的积累并触发基于 SA 的防御机制。在转基因植物中，SA 响应元件 表现出更高的表达，从而限制了病原体在植物组织中的生长。这些结果表明 StRbohA 在增加土豆对 的防御中具有有效作用。
