Schwartz J L, Giovanazzi S M, Karrison T, Jones C, Grdina D J
Department of Radiation Oncology, University of Chicago Medical Center, Illinois 60637.
Radiat Res. 1988 Jan;113(1):145-54.
The radioprotector 2-[aminopropyl)amino] ethanethiol (WR1065), which has been reported to reduce the cytotoxic and mutagenic effects of low LET radiation, was investigated for its ability to protect against low LET (60Co gamma ray) and high LET (fission-spectrum neutron)-induced chromosome damage in V79 cells. Cells were irradiated in G2 phase in the presence or absence of 4 mM WR1065 and were harvested and analyzed 2 h later for chromatid-type aberrations. Irradiation of G2-phase V79 cells in the presence of WR1065 resulted in a 30 to 50% reduction in the frequency of gamma-ray and neutron-induced chromatid-type breaks and exchanges. The effects were found only after exposures of greater than 200 cGy gamma-ray or 50 cGy neutron irradiation. The radioprotector was effective at reducing neutron-induced aberrations after exposures at dose rates of both 10 and 43 cGy/min. Thus the radioprotector WR1065 is an effective anti-clastogenic agent in V79 cells, protecting against both 60Co gamma-ray and fission-spectrum neutron-induced aberrations, when present during irradiation.
放射防护剂2-[(氨丙基)氨基]乙硫醇（WR1065）已被报道可降低低传能线密度辐射的细胞毒性和诱变作用，本研究对其在V79细胞中抵御低传能线密度（60Coγ射线）和高传能线密度（裂变谱中子）诱导的染色体损伤的能力进行了研究。细胞在G2期进行辐照，辐照时存在或不存在4 mM WR1065，2小时后收获细胞并分析染色单体型畸变。在WR1065存在的情况下对G2期V79细胞进行辐照，γ射线和中子诱导的染色单体型断裂和交换频率降低了30%至50%。仅在大于200 cGy的γ射线或50 cGy的中子辐照后才发现这种效果。在10和43 cGy/min的剂量率下进行辐照后，该放射防护剂在降低中子诱导的畸变方面均有效。因此，放射防护剂WR1065在V79细胞中是一种有效的抗断裂剂，在辐照期间存在时，可抵御60Coγ射线和裂变谱中子诱导的畸变。
