Song Qing-Hua, Guo Ming-Jun, Zheng Jun-Shui, Zheng Xue-Hong, Ye Zhao-Hui, Wei Peng
Department of Repair and Reconstruction Surgery, The First Hospital of Ningbo, Ningbo, Zhejiang Province 315000, People's Republic of China.
Medical College, Ningbo University, Ningbo, Zhejiang Province 315000, People's Republic of China.
Cancer Manag Res. 2020 Dec 31;12:13589-13598. doi: 10.2147/CMAR.S264682. eCollection 2020.
To probe into the expression of FGD5-AS1 in osteosarcoma and its relationship with miR-320b.
The tissue and serum samples of 97 patients with osteosarcoma were collected, and the serum samples of 100 healthy subjects who concurrently underwent physical examination were selected as the control. FGD5-AS1 expression in tissues and serum was detected, and osteosarcoma cells were transfected to measure cell behaviors such as proliferation, invasion and apoptosis.
FGD5-AS1 was highly expressed in osteosarcoma, and its elevated expression indicated poor survival of patients. Serum FGD5-AS1 was related to tumor size and clinical stage and could be used for the diagnosis of osteosarcoma. The study of osteosarcoma cell lines U2OS and SaOS-2 showed that after inhibiting FGD5-AS1, the viability and invasion capacity of osteosarcoma cells decreased statistically compared with the control group (CG), while the apoptosis ability could be improved by further regulating apoptotic proteins (P<0.05). Detection of EMT-related proteins identified that E-cadherin increased while N-cadherin decreased significantly after FGD5-AS1 inhibition (P<0.05). Correlation analysis revealed a negative correlation between miR-320b and FGD5-AS1 (r = -0.410, P<0.001). Overexpression of miR-320b significantly inhibited cell viability, invasion and EMT ability, and increased the apoptosis rate, while inhibiting miR-320b expression produced the opposite results. The targeting relationship between miR-320b and FGD5-AS1 was confirmed through the biological prediction website, luciferase assay and RNA binding protein immunoprecipitation (RIP) assay. Inhibition of miR-320b could reverse the regulatory effect of FGD5-AS1 knockdown on osteosarcoma cells.
FGD5-AS1 is highly expressed in osteosarcoma and is involved in the biological procession of osteosarcoma by targeting miR-320b.
探讨FGD5-AS1在骨肉瘤中的表达及其与miR-320b的关系。
收集97例骨肉瘤患者的组织和血清样本,并选取100例同期进行体检的健康受试者的血清样本作为对照。检测组织和血清中FGD5-AS1的表达,并对骨肉瘤细胞进行转染以检测细胞增殖、侵袭和凋亡等行为。
FGD5-AS1在骨肉瘤中高表达,其表达升高提示患者生存预后较差。血清FGD5-AS1与肿瘤大小和临床分期相关,可用于骨肉瘤的诊断。对骨肉瘤细胞系U2OS和SaOS-2的研究表明,抑制FGD5-AS1后,骨肉瘤细胞的活力和侵袭能力与对照组相比有统计学意义的下降,而通过进一步调节凋亡蛋白可提高凋亡能力(P<0.05)。检测EMT相关蛋白发现,抑制FGD5-AS1后E-钙黏蛋白增加而N-钙黏蛋白显著降低(P<0.05)。相关性分析显示miR-320b与FGD5-AS1呈负相关(r = -0.410,P<0.001)。miR-320b过表达显著抑制细胞活力、侵袭和EMT能力,并增加凋亡率,而抑制miR-320b表达则产生相反结果。通过生物预测网站、荧光素酶报告基因检测和RNA结合蛋白免疫沉淀(RIP)检测证实了miR-320b与FGD5-AS1之间的靶向关系。抑制miR-320b可逆转FGD5-AS1敲低对骨肉瘤细胞的调节作用。
FGD5-AS1在骨肉瘤中高表达,并通过靶向miR-320b参与骨肉瘤的生物学进程。