Department of Endocrinology, People's Hospital of Rizhao, Shandong, China.
Department of Cardiology, Affiliated Hospital of Hebei University, Hebei, China.
Bioengineered. 2022 Feb;13(2):2648-2657. doi: 10.1080/21655979.2021.2023995.
Study shows that long non-coding RNA (lncRNA) plays a regulatory role in cardiovascular diseases, and the mechanism of rhabdomyosarcoma 2-associated transcript (RMST) in atherosclerosis (AS) is still unclear. This study aimed to evaluate the expression of RMST and its possible role in the occurrence of AS. RMST and miR-224-3p level in serum and human umbilical vein endothelial cells (HUVECs) were determined by real-time quantitative PCR (RT-qPCR). In vitro atherosclerotic cell model was achieved by treating HUVECs with ox-LDL. Receiver operating characteristic (ROC) curve assessed the diagnostic value of RMST in AS, and Pearson correlation coefficient estimated the correlation of RMST with carotid intima-media thickness (CIMT) and carotid-femoral pulse wave velocity (cfPWV). Cell counting kit-8 (CCK-8) assay and Enzyme-linked immunosorbent assay (ELISA) were performed to evaluate the effect of RMST on cell viability and inflammatory response. The luciferase analysis was used to validate the relationship between RMST and miR-224-3p. The results showed that in serum and HUVECs, RMST levels were increased, while miR-224-3p level was decreased. ROC curve suggested that RMST had clinical diagnostic value for AS. Besides, CIMT and cfPWV were positively correlated with RMST levels, respectively. In HUVECs, RMST-knockdown notably improved the cell viability and inhibited the production of inflammatory factors. Moreover, miR-224-3p was the target of RMST. In conclusion, RMST has the potential to be a diagnostic marker for AS. RMST-knockdown contributes to the enhancement of cell viability and the inhibition of inflammatory response, which may provide new insights into the conquest of AS.
研究表明，长链非编码 RNA（lncRNA）在心血管疾病中发挥着调节作用，而横纹肌肉瘤 2 相关转录物（RMST）在动脉粥样硬化（AS）中的机制尚不清楚。本研究旨在评估 RMST 的表达及其在 AS 发生中的可能作用。通过实时定量 PCR（RT-qPCR）测定血清和人脐静脉内皮细胞（HUVEC）中的 RMST 和 miR-224-3p 水平。用 ox-LDL 处理 HUVEC 以建立体外动脉粥样硬化细胞模型。接受者操作特征（ROC）曲线评估 RMST 在 AS 中的诊断价值，Pearson 相关系数估计 RMST 与颈动脉内膜中层厚度（CIMT）和颈动脉-股动脉脉搏波速度（cfPWV）的相关性。细胞计数试剂盒-8（CCK-8）测定和酶联免疫吸附测定（ELISA）用于评估 RMST 对细胞活力和炎症反应的影响。荧光素酶分析用于验证 RMST 与 miR-224-3p 的关系。结果表明，血清和 HUVEC 中 RMST 水平升高，而 miR-224-3p 水平降低。ROC 曲线提示 RMST 对 AS 具有临床诊断价值。此外，CIMT 和 cfPWV 分别与 RMST 水平呈正相关。在 HUVEC 中，RMST 敲低显著提高了细胞活力并抑制了炎症因子的产生。此外，miR-224-3p 是 RMST 的靶标。总之，RMST 有可能成为 AS 的诊断标志物。RMST 敲低有助于增强细胞活力和抑制炎症反应，这可能为征服 AS 提供新的思路。
