Department of Veterinary Experimental Animals, Faculty of Veterinary Medicine, Ondokuz Mayis University, 55139, Atakum, Samsun, Turkey.
Bornova Veterinary Control Institute, Veterinary Control Institute Directorates, Ministry of Agriculture and Forestry, 35010, Izmir, Turkey.
Arch Virol. 2021 Feb;166(2):559-569. doi: 10.1007/s00705-020-04943-6. Epub 2021 Jan 6.
Marek's disease (MD) is an important disease of avian species and a potential threat to the poultry industry worldwide. In this study, 16 dead commercial chickens from flocks with suspected MD were necropsied immediately after death. Pathological findings were compatible with MD, and gallid alphaherpesvirus 2 was identified in PCR of spleen samples. Virus isolation was performed in primary cell culture, and partial sequencing of the meq gene of the isolate revealed >99% nucleotide sequence identity to virulent and very virulent plus strains from a number of European countries, placing it in the same subclade of clade III as two virulent Italian strains and a very virulent plus Polish strain as well as virulent strains of geese and ducks. The data reported here indicate that a virulent strain of Marek's disease virus is circulating in Turkey and has not been stopped by the current national vaccination programme.
马立克氏病（MD）是一种重要的禽类疾病，也是全球家禽业的潜在威胁。在本研究中，16 只来自疑似 MD 鸡群的死亡商业鸡在死后立即进行剖检。病理学发现与 MD 相符，并且在脾脏样本的 PCR 中鉴定出了禽α疱疹病毒 2。在原代细胞培养中进行了病毒分离，分离株的 meq 基因的部分测序显示与来自多个欧洲国家的强毒和超强毒株的核苷酸序列同一性>99%，将其与两个意大利强毒株和一个波兰超强毒株以及鹅和鸭的强毒株一起置于 III 组的同一个亚分支中。本报告的数据表明，一种强毒马立克氏病病毒正在土耳其流行，目前的国家疫苗接种计划并未阻止该病毒的传播。
