Vesilind G W, Simpson M B, Shifman M A, Colman R E, Kao K J
Transfusion Service, Duke University Medical Center, Durham, North Carolina.
Transfusion. 1988 Jan-Feb;28(1):46-51. doi: 10.1046/j.1537-2995.1988.28188127952.x.
A semiautomated saline wash procedure using a blood cell processor was evaluated as a technique for removing plasma from platelet concentrates. In vitro studies demonstrated 92 to 99.6 percent (mean, 96%) removal of total plasma protein (n = 30) with 84 to 97 percent (mean, 90.8%) platelet recovery (n = 28) in post-wash units. Post-wash pH values changed by +0.2 to -0.86 (mean, -0.47) (n = 30); the level of recovery from hypotonic shock was 69 to 97 percent (mean, 86%) (n = 11) of pre-wash units; weighted morphology scores decreased from a mean of 248 to 223 (n = 9). Aggregation response to arachidonic acid, collagen, and adenosine diphosphate plus epinephrine showed essentially no change following the wash procedure, and electron microscopy demonstrated slight morphologic alteration. Autologous platelets labeled with indium-111 demonstrated 43 +/- 20 percent recovery (n = 11) for washed units, compared to 41 +/- 10 percent for control unwashed units (n = 5); mean survivals were 140 +/- 41 hours (n = 11) for washed platelets and 185 +/- 28 hours for unwashed units (n = 5). Thirteen alloimmunized patients receiving 55 washed platelet concentrates demonstrated a mean 1- to 4-hour corrected count increment of 3.99 X 10(3) per microliter, compared to 3.02 X 10(3) per microliter for 77 unwashed platelet units given to the same patients. This study documents that platelet concentrates maintain viability and efficacy following a semiautomated saline wash method using the Cobe 2991 Blood Cell Processor, a technique that may be helpful for patients who require plasma-depleted platelet transfusions.
一种使用血细胞处理器的半自动盐水洗涤程序被评估为一种从血小板浓缩物中去除血浆的技术。体外研究表明，洗涤后单位中总血浆蛋白的去除率为92%至99.6%（平均96%）（n = 30），血小板回收率为84%至97%（平均90.8%）（n = 28）。洗涤后pH值变化为+0.2至-0.86（平均-0.47）（n = 30）；低渗休克恢复水平为洗涤前单位的69%至97%（平均86%）（n = 11）；加权形态学评分从平均248降至223（n = 9）。洗涤程序后，对花生四烯酸、胶原蛋白以及二磷酸腺苷加肾上腺素的聚集反应基本没有变化，电子显微镜显示形态有轻微改变。用铟-111标记的自体血小板在洗涤单位中的回收率为43±20%（n = 11），而对照未洗涤单位的回收率为41±10%（n = 5）；洗涤血小板的平均存活时间为140±41小时（n = 11），未洗涤单位为185±28小时（n = 5）。13名接受55个洗涤血小板浓缩物的同种免疫患者，其平均1至4小时校正计数增加值为每微升3.99×10³，而给予这些患者的77个未洗涤血小板单位的该值为每微升3.02×10³。这项研究证明，使用Cobe 2991血细胞处理器的半自动盐水洗涤方法后，血小板浓缩物仍保持活力和功效，该技术可能对需要输注去除血浆的血小板的患者有帮助。
