Ashland Specialties France, Global Skin Research Center, Nice 06904, France (F.L., A.P., Y.F., G.O., C.G., C.M., K.C.).
J Cosmet Sci. 2020 Nov-Dec;71(6):425-437.
Skin functions as a neuro-immuno-endocrine tissue with well-defined neuronal networks and functions. The endocannabinoid system has been proven to be an important, homeostatic regulator for homeostatic and inflammatory events. The system comprises endogenous or exogenous ligands and receptors (CB1 and CB2). In the present study, we evaluated the soothing properties of a (patchouli) extract. Agonist AM1241 and antagonist AM630 were used for CB2 receptor activation/inhibition. Expression of CB2 receptor and β-endorphin was monitored by immunohistochemistry. Skin inflammation was induced with ultraviolet B (UVB) or lipopolysaccharide (LPS), and the following markers were used to highlight the anti-inflammatory properties of the extract: transient receptor potential vanilloid 1 (TRPV1), interleukin receptors 1 (IL1R1), and the interleukin 6 signal transducer (IL6ST). Our results demonstrated the implication of the CB2 receptor in the skin inflammation process. The expression of CB2 receptor and β-endorphin was increased 48 hours after application of the extract. Furthermore, patchouli extract application helped to reduce IL1R1, IL6ST, and TRPV1 expression, in skin exposed to UVB or LPS. In conclusion, the application of the patchouli extract helps maintain skin integrity and reduce skin discomfort modulation of CB2 receptor stimulation and the subsequent β-endorphin release.
皮肤作为一种具有明确神经元网络和功能的神经免疫内分泌组织。内源性大麻素系统已被证明是一种重要的、维持内环境稳定的调节剂，可调节内环境稳定和炎症事件。该系统包括内源性或外源性配体和受体（CB1 和 CB2）。在本研究中，我们评估了 （广藿香）提取物的舒缓特性。激动剂 AM1241 和拮抗剂 AM630 用于 CB2 受体的激活/抑制。通过免疫组织化学监测 CB2 受体和 β-内啡肽的表达。使用紫外线 B（UVB）或脂多糖（LPS）诱导皮肤炎症，并使用以下标志物突出提取物的抗炎特性：瞬时受体电位香草酸 1（TRPV1）、白细胞介素受体 1（IL1R1）和白细胞介素 6 信号转导物（IL6ST）。我们的结果表明 CB2 受体参与了皮肤炎症过程。在提取物应用 48 小时后，CB2 受体和 β-内啡肽的表达增加。此外，广藿香提取物的应用有助于减少 IL1R1、IL6ST 和 TRPV1 的表达，在暴露于 UVB 或 LPS 的皮肤中。总之，广藿香提取物的应用有助于维持皮肤完整性并减轻皮肤不适，通过调节 CB2 受体的刺激和随后的 β-内啡肽释放。
