Liu Binghua, Liu Xinghong, Liu Fangchun, Ma Hailin, Ma Bingyao, Peng Lin
Shandong Academy of Forestry, 42, East Wenhua Road, Shandong, 250014, Jinan, China.
Economic Forest Products Quality Inspection Test Center of State Forestry Administration (Jinan), Shandong, 250014, Jinan, China.
AMB Express. 2021 Jan 7;11(1):15. doi: 10.1186/s13568-020-01172-7.
Comparative evaluations were conducted to assess the effects of different pH levels, NaCl-induced salt stress, and PEG-induced drought stress on the mycelial growth of Xerocomus badius. The results showed that X. badius mycelium grew well at a wide pH range of 5.00 ~ 9.00. Although the mycelium remained viable, mycelial growth of X. badius was significantly inhibited with increasing salt and drought stresses. Furthermore, a soilless experiment in Petri dishes was performed to investigate the potential of X. badius to induce beneficial effects on seed germination and seedling growth of annual ryegrass (Lolium multiflorum Lam.) under salt and drought stresses. Seed priming with X. badius enhanced the seedling growth of L. multiflorum Lam. under NaCl-induced salt stress and PEG-induced drought stress. However, X. badius did not significantly improve the seed germination under non-stress and mild stress conditions. It suggested that X. badius inoculation with seeds was not essential for seed germination under non-stress and mild stress conditions, but contributed highly to seedling growth under severe stress conditions. Therefore, seed priming with X. badius on ryegrass could be an effective approach to enhance plant tolerance against drought and salt stresses. X. badius could be a good candidate for the inoculation of ectomycorrhizal plants cultivation programs in mild saline and semiarid areas.
进行了比较评估，以评估不同pH值水平、NaCl诱导的盐胁迫和PEG诱导的干旱胁迫对褐绒盖牛肝菌菌丝体生长的影响。结果表明，褐绒盖牛肝菌菌丝体在5.00至9.00的宽pH范围内生长良好。尽管菌丝体仍具活力，但随着盐胁迫和干旱胁迫的增加，褐绒盖牛肝菌的菌丝体生长受到显著抑制。此外，在培养皿中进行了无土实验，以研究褐绒盖牛肝菌在盐胁迫和干旱胁迫下对一年生黑麦草（多花黑麦草）种子萌发和幼苗生长产生有益影响的潜力。用褐绒盖牛肝菌进行种子引发可增强多花黑麦草在NaCl诱导的盐胁迫和PEG诱导的干旱胁迫下的幼苗生长。然而，在非胁迫和轻度胁迫条件下，褐绒盖牛肝菌并未显著提高种子发芽率。这表明在非胁迫和轻度胁迫条件下，用褐绒盖牛菌接种种子对种子萌发并非必不可少，但在重度胁迫条件下对幼苗生长有很大贡献。因此，用褐绒盖牛肝菌对黑麦草进行种子引发可能是提高植物抗旱和耐盐胁迫能力的有效方法。褐绒盖牛肝菌可能是轻度盐碱地和半干旱地区外生菌根植物栽培接种的良好候选菌种。
