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利用源自胰蛋白酶-1 的信号肽-前肽结构域提高 HEK293 细胞中重组人 IL-25 的分泌。

Improved secretion of recombinant human IL-25 in HEK293 cells using a signal peptide-pro-peptide domain derived from Trypsin-1.

机构信息

Protein Technologies Group, GlaxoSmithKline Research & Development, Gunnels Wood Road, Stevenage, SG1 2NY, Hertfordshire, UK.

Department of Protein & Cellular Sciences, GlaxoSmithKline Research & Development, Gunnels Wood Road, Stevenage, SG1 2NY, Hertfordshire, UK.

出版信息

Biotechnol Lett. 2021 Apr;43(4):757-765. doi: 10.1007/s10529-020-03072-z. Epub 2021 Jan 7.

DOI:10.1007/s10529-020-03072-z
PMID:33415569
Abstract

OBJECTIVE

To compare the effects of human Trypsin-1 signal peptide and pro-peptide on the expression and secretion efficiency of human Interleukin-25 from mammalian cells.

RESULTS

The signal peptide and combined signal peptide-pro-peptide sequence of human Trypsin-1 improved the secretion of human IL-25 from 1.7 to 3.2 µg/ml and 1.7 to 8.2 µg/ml, respectively. Deletion analysis identified the minimal Trypsin-1 derived secretion domain that maintains improved human Interleukin-25 production and secretion. The presence of Trypsin-1 pro-peptide sequence does not affect the function of secreted human Interleukin-25.

CONCLUSION

The Trypsin-1 signal peptide-pro-peptide sequence increased human IL-25 expression and secretion in mammalian cells by fivefold.

摘要

目的

比较人胰蛋白酶 1 信号肽和前肽对人白细胞介素 25 在哺乳动物细胞中表达和分泌效率的影响。

结果

人胰蛋白酶 1 的信号肽和信号肽-前肽序列分别将人白细胞介素 25 的分泌量从 1.7µg/ml 提高到 3.2µg/ml 和 1.7µg/ml 提高到 8.2µg/ml。缺失分析确定了保持提高的人白细胞介素 25 产生和分泌的最小胰蛋白酶 1 衍生分泌结构域。前肽序列的存在并不影响分泌的人白细胞介素 25 的功能。

结论

胰蛋白酶 1 信号肽-前肽序列将人白细胞介素 25 在哺乳动物细胞中的表达和分泌提高了五倍。

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2
A comprehensive review of signal peptides: Structure, roles, and applications.信号肽的综合综述：结构、作用与应用。
Eur J Cell Biol. 2018 Aug;97(6):422-441. doi: 10.1016/j.ejcb.2018.06.003. Epub 2018 Jun 22.
3
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4
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Respir Res. 2016 Jan 14;17:5. doi: 10.1186/s12931-016-0321-z.
5
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Immunity. 2015 Apr 21;42(4):692-703. doi: 10.1016/j.immuni.2015.03.008. Epub 2015 Apr 14.
6
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Science. 2015 Jan 23;347(6220):1260419. doi: 10.1126/science.1260419.
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