Department of Respiratory and Critical Care Medicine, Wuxi Fifth People's Hospital, Wuxi, Jiangsu 214000, P.R. China.
Department of Nuclear Medicine, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu 213003, P.R. China.
Int J Mol Med. 2021 Feb;47(2):523-532. doi: 10.3892/ijmm.2020.4816. Epub 2020 Dec 14.
Previous studies have reported that long non‑coding (lnc) RNA FGD5‑antisense 1 (FGD5‑AS1) promotes tumor proliferation, migration and invasion. Therefore, the present study aimed to elucidate the biological role and underlying molecular mechanisms of FGD5‑AS1 in cisplatin (DDP) resistance of lung adenocarcinoma (LAD) cells. The results demonstrated that FGD5‑AS1 was highly expressed in DDP‑resistant LAD tissues and cells. Knockdown of FGD5‑AS1 decreased the proliferative, migratory and invasive abilities of DDP‑resistant LAD cells. Moreover, it was identified that FGD5‑AS1 acted as a molecular sponge for microRNA (miR)‑142, and FGD5‑AS1 enhanced the resistance of A549/DDP cells to DDP by directly interacting with miR‑142. Programmed cell death 1 ligand 1 (PD‑L1) was also found to be a key effector of the FGD5‑AS1/miR‑142 axis to regulate the chemoresistance of DDP‑resistant LAD cells. In conclusion, the present study demonstrated that FGD5‑AS1 increased DDP resistance of LAD via the miR‑142/PD‑L1 axis, which may offer a novel treatment strategy for patients with DDP‑resistant LAD.
先前的研究报告称，长链非编码（lnc）RNA FGD5-反义 1（FGD5-AS1）可促进肿瘤的增殖、迁移和侵袭。因此，本研究旨在阐明 FGD5-AS1 在顺铂（DDP）耐药肺腺癌（LAD）细胞中的生物学作用和潜在分子机制。结果表明，FGD5-AS1 在 DDP 耐药 LAD 组织和细胞中呈高表达。FGD5-AS1 敲低可降低 DDP 耐药 LAD 细胞的增殖、迁移和侵袭能力。此外，研究还发现，FGD5-AS1 作为 microRNA（miR）-142 的分子海绵，通过与 miR-142 直接相互作用增强 A549/DDP 细胞对 DDP 的耐药性。程序性细胞死亡配体 1（PD-L1）也被发现是 FGD5-AS1/miR-142 轴的关键效应因子，可调节 DDP 耐药 LAD 细胞的化疗耐药性。综上所述，本研究表明，FGD5-AS1 通过 miR-142/PD-L1 轴增加 LAD 对 DDP 的耐药性，这可能为 DDP 耐药 LAD 患者提供一种新的治疗策略。
