School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, China.
Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China.
Anal Chem. 2021 Feb 2;93(4):2160-2165. doi: 10.1021/acs.analchem.0c03975. Epub 2021 Jan 8.
Screening toxic-element-free and biocompatible electrochemiluminophores was crucial for electrochemiluminescence (ECL) evolution. Herein, l-glutathione (GSH)-capped Ag-Ga-In-S (AGIS) nanocrystals (NCs) were prepared by doping Ag-In-S (AIS) NCs in a doping-in-growth way and utilized as a model for both ECL modulating and developing multinary NC-based electrochemiluminophores with enhanced ECL performance than trinary NCs. AGIS NCs not only primarily preserved the morphology, size, phase structure, and water monodisperse characteristics of AIS NCs with broadened band gap but also demonstrated obviously enhanced oxidative-reduction ECL than AIS NCs. Importantly, ECL of AGIS NCs was located at the near-infrared region with a maximum emission wavelength of 744 nm and could be utilized for an ECL immunoassay with human prostate-specific antigen (PSA) as a model, which exhibited a linearity range from 0.05 pg/mL to 1.0 ng/mL and a low limit of detection of 0.01 pg/mL (S/N = 3). This work provided a promising alternative to the traditional binary NCs for developing toxic-element-free and biocompatible electrochemiluminophores with efficient near-infrared ECL.
筛选无毒性元素且生物相容的电化学发光体对于电化学发光（ECL）的发展至关重要。在此，通过掺杂生长的方式将 Ag-In-S（AIS）NCs 掺杂到 l-谷胱甘肽（GSH）包覆的 Ag-Ga-In-S（AGIS）NCs 中，制备了作为模型的 GSH 包覆的 Ag-Ga-In-S（AGIS）NCs，用于调节和开发基于多元 NC 的电化学发光体，与三元 NCs 相比，具有增强的 ECL 性能。AGIS NCs 不仅主要保留了 AIS NCs 的形态、尺寸、相结构和宽的带隙水单分散特性，而且表现出比 AIS NCs 明显增强的氧化还原 ECL。重要的是，AGIS NCs 的 ECL 位于近红外区域，最大发射波长为 744nm，可用于以人前列腺特异性抗原（PSA）为模型的 ECL 免疫分析，其线性范围为 0.05pg/mL 至 1.0ng/mL，检测限低至 0.01pg/mL（S/N=3）。这项工作为开发无毒性元素且生物相容的具有高效近红外 ECL 的电化学发光体提供了一种有前景的替代传统二元 NCs 的方法。
