College of Animal Sciences and Technology, Northeast Agricultural University, Harbin, 150030, China.
College of Life Sciences, Northeast Agricultural University, Harbin, 150030, China.
Plant Physiol Biochem. 2021 Feb;159:301-311. doi: 10.1016/j.plaphy.2020.12.021. Epub 2021 Jan 6.
Alkaline salt is one of the most devastating environmental factors limiting alfalfa productivity, however, the mechanisms underlying adaptation of alfalfa to alkaline remain unclear. Our aim is to investigate proteomic and metabolomic differences in growth and root of alfalfa under alkaline salt in Rhizobium-alfalfa symbiotic relationships.
Rhizobium-inoculated and non-inoculated alfalfa plants were treated with 200 mmol/L NaHCO to investigate physiological, metabolic, and proteomic responses of root-nodule symbiosis under alkaline-induced stress, using an integrated approach combining metabolome and proteome analysis with measurements of physiological parameters.
The improved tolerance to alkalinity was observed in RI-plants compared with NI-plants. RI-plants accumulated more proline and MDH, and had higher antioxidant activity and relatively high RWC but low MDA content and low Na/K ratio. The stress-related genes (P5CS, GST13, H-Ppase, NADP-Me, SDH, and CS) were actively upregulated in RI plants under alkaline stress. In RI-plants, damage caused by alkaline stress was mainly alleviated by decreasing oxidative damage, enhancing the organic acid and amino acid metabolic processes, and scavenging harmful ROS by activating the phenylpropanoid biosynthetic pathway.
We revealed distinct proteins and metabolites related to alkali tolerance in RI-plants compared to NI-plants. Alkali tolerance of rhizobia-inoculated alfalfa was enhanced by altered proteins and metabolic processes as well as decreased oxidative damage.
碱性盐是限制苜蓿生产力的最具破坏性的环境因素之一，但苜蓿适应碱性的机制尚不清楚。我们的目的是研究在根瘤菌-苜蓿共生关系中，碱性盐胁迫下苜蓿生长和根系的蛋白质组学和代谢组学差异。
用 200mmol/L NaHCO3 处理接种和未接种根瘤菌的苜蓿植物，以研究碱性诱导胁迫下根瘤-共生体的生理、代谢和蛋白质组学反应，采用代谢组学和蛋白质组学分析与生理参数测量相结合的综合方法。
与未接种根瘤菌的植物相比，接种根瘤菌的植物对碱性的耐受性有所提高。接种根瘤菌的植物积累了更多的脯氨酸和 MDH，具有更高的抗氧化活性和相对较高的 RWC，但 MDA 含量和 Na/K 比值较低。在碱性胁迫下，与应激相关的基因（P5CS、GST13、H-Ppase、NADP-Me、SDH 和 CS）在 RI 植物中被积极上调。在 RI 植物中，碱性胁迫引起的损伤主要通过降低氧化损伤、增强有机酸和氨基酸代谢过程以及通过激活苯丙烷生物合成途径清除有害的 ROS 来缓解。
与未接种根瘤菌的植物相比，我们在接种根瘤菌的植物中发现了与耐碱相关的明显的蛋白质和代谢物。接种根瘤菌的苜蓿的耐碱性通过改变蛋白质和代谢过程以及降低氧化损伤来增强。
