Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, No. 1 of Shida Road, Limin Development, China.
Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, No. 1 of Shida Road, Limin Development, China.
Plant Physiol Biochem. 2022 Jan 1;170:146-159. doi: 10.1016/j.plaphy.2021.12.003. Epub 2021 Dec 5.
Saline-alkali stress is a major abiotic stress that limits plant growth, yield, and geographical distribution. Alfalfa is a perennial legume with the largest planting area in the world because of its high protein content, good palatability, and long utilization life. However, saline-alkali stress seriously affects alfalfa yield and quality. To better understand the saline-alkali stress response mechanisms of alfalfa, an isobaric tags proteomics method was used to compare and analyse alfalfa under saline-alkali stress for 0, 1, and 7 days, and 126 (1 vs. 0 days) and 1869 (7 vs. 0 days) differentially abundant proteins (DAPs) were found. Through integrative analysis with differentially expressed genes (DEGs), we found correlated DEGs-DAPs of RNA and protein with similar expression trends at the mRNA and protein levels; these were mainly involved in ABA and Ca signal pathways, regulation of photosynthesis, ROS scavenging, secondary metabolism, and transcription factors (TFs) related to saline-alkali stress. Some genes not exhibiting such trends may have been regulated post-transcriptionally. Furthermore, through transgenic experiments, MsFTL was found to significantly improve the saline-alkali tolerance of plants. Overall, our findings provide important clues for understanding the molecular mechanisms underlying the response of alfalfa to saline-alkali stress.
盐胁迫是限制植物生长、产量和地理分布的主要非生物胁迫之一。紫花苜蓿是一种多年生豆科植物，由于其高蛋白含量、良好的适口性和较长的利用寿命，是世界上种植面积最大的植物。然而，盐胁迫严重影响紫花苜蓿的产量和品质。为了更好地了解紫花苜蓿对盐胁迫的响应机制，采用等压标签蛋白质组学方法比较分析了盐胁迫下的紫花苜蓿，发现了 126 个（1 天与 0 天相比）和 1869 个（7 天与 0 天相比）差异丰度蛋白（DAP）。通过与差异表达基因（DEGs）的综合分析，我们发现了 RNA 和蛋白质的相关 DEGs-DAPs，它们在 mRNA 和蛋白质水平上具有相似的表达趋势；这些 DAPs 主要涉及 ABA 和 Ca 信号通路、光合作用的调节、ROS 清除、次生代谢和与盐胁迫相关的转录因子（TFs）。一些不表现出这种趋势的基因可能在转录后受到调控。此外，通过转基因实验发现 MsFTL 显著提高了植物的耐盐碱性。总的来说，我们的研究结果为理解紫花苜蓿对盐胁迫响应的分子机制提供了重要线索。
