十株柯萨奇病毒 A10 病毒株生物学特性的比较研究。

A comparative study on biological characteristics of ten coxsackievirus A10 virus strains.

机构信息

Institute of Medical Biology, Chinese Academy of Medical Sciences, China.

Institute of Medical Biology, Chinese Academy of Medical Sciences, China; Key Laboratory for Vaccine Research and Development of Major Infectious Diseases in Yunnan Province, Kunming, Yunnan, 650118, China.

出版信息

Virology. 2021 Mar;555:1-9. doi: 10.1016/j.virol.2020.07.003. Epub 2020 Aug 17.

DOI:10.1016/j.virol.2020.07.003

PMID:33418337

Abstract

In this study, we analyzed ten CVA10 strains were genotyped and cultured for 10 generations to detect plaque morphology, pathogenicity, growth and other characteristics. Mice were injected with live and inactivated virus to detect neutralizing antibody titers. The results suggested that all CVA10 strains fell into Genotype C. Each strain cultured on KMB and Vero cells, increased from 1st generation onwards to peak in the 3rd and 4th, and the titer at which each became infectious ranged from 5.0 to 6.5 and 6.0 to 7.0 lgCCID/ml, respectively. Two-day-old BALB/c mice were selected and inoculated intracerebral with the CVA10 strains, Limb paralysis was significant as early as 3 d; paralysis of all limbs for 50% of affected mice. LT was approximately 6 d, all died within 8 d. Ten strains induced good immune response, the GMT value of booster immunizations was higher than that of initial immunization. This provide reference points for selecting CVA10 vaccine candidates.

摘要

在这项研究中，我们分析了 10 株 CVA10 病毒株，对其进行了 10 代的基因分型和培养，以检测其斑块形态、致病性、生长等特征。我们用活病毒和灭活病毒对小鼠进行了注射，以检测中和抗体滴度。结果表明，所有 CVA10 病毒株均属于基因型 C。每种病毒株均在 KMB 和 Vero 细胞上培养，从第一代开始增加，到第 3 代和第 4 代达到峰值，每个病毒株的感染滴度分别为 5.0 至 6.5 和 6.0 至 7.0 lgCCID/ml。我们选择 2 天大的 BALB/c 小鼠，通过脑内接种 CVA10 病毒株进行感染，感染后 3 天就出现明显的肢体瘫痪；50%的受感染小鼠出现四肢瘫痪。LT 约为 6 天，所有小鼠均在 8 天内死亡。这 10 株病毒株均可诱导良好的免疫反应，加强免疫后的 GMT 值高于初次免疫。这些结果为选择 CVA10 疫苗候选株提供了参考依据。