低氧对头颈部鳞状细胞癌细胞系增殖及 HIF-1α 和 JMJD1A 基因表达的影响。

Effect of Hypoxia on Proliferation and the Expression of the Genes HIF-1α and JMJD1A in Head and Neck Squamous Cell Carcinoma Cell Lines.

机构信息

Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Julius Maximilian University of Wuerzburg, Wuerzburg, Germany

Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery, Julius Maximilian University of Wuerzburg, Wuerzburg, Germany.

出版信息

Anticancer Res. 2021 Jan;41(1):113-122. doi: 10.21873/anticanres.14756.

DOI:10.21873/anticanres.14756

PMID:33419804

Abstract

BACKGROUND/AIM: The aim of the study was to investigate the effects of hypoxia on proliferation and the expression of HIF-1α (hypoxia-inducible factor 1 alpha) and JMJD1A (jumonji domain 1A) in head and neck squamous cell carcinoma (HNSCC).

MATERIALS AND METHODS

FaDu and HLaC78 cells were incubated for 1-24 h in hypoxia and normoxia. Cell proliferation, mRNA and protein levels of HIF-1α and JMJD1A were quantified by counting, PCR and western blot.

RESULTS

Hypoxia led to a constant decrease in cell proliferation. Short hypoxia resulted in an increase in HIF-1α mRNA levels. This effect was reversed after longer incubation. The western blot for HIF-1α showed a maximum accumulation after 3-6 h of hypoxia. In FaDu cells, the concentration of JMJD1A reached a peak after 6 h and decreased thereafter, whereas in HLaC78 cells, it presented a second peak after 48 h.

CONCLUSION

The transcription factors HIF-1α and JMJDA1 were confirmed as relevant hypoxia-dependent regulators of carcinogenesis in HNSCC.

摘要

背景/目的：本研究旨在探讨低氧对头颈部鳞状细胞癌（HNSCC）增殖以及缺氧诱导因子 1α（HIF-1α）和 JMJD1A（jumonji 结构域 1A）表达的影响。

材料和方法

将 FaDu 和 HLaC78 细胞在低氧和常氧条件下孵育 1-24 小时。通过计数、PCR 和 Western blot 定量检测 HIF-1α 和 JMJD1A 的细胞增殖、mRNA 和蛋白水平。

结果

低氧导致细胞增殖持续减少。短暂的低氧导致 HIF-1α mRNA 水平增加。这种作用在更长时间的孵育后被逆转。HIF-1α 的 Western blot 显示在低氧 3-6 小时后达到最大积累。在 FaDu 细胞中，JMJD1A 的浓度在 6 小时后达到峰值，随后下降，而在 HLaC78 细胞中，48 小时后出现第二个峰值。