University Hospital Heidelberg, Center for Orthopedics, Trauma surgery and Paraplegiology, Clinic for Orthopedics and Trauma surgery, Schlierbacher Landstrasse 200 A, 69118, Heidelberg, Germany.
Department of Nuclear Medicine, University Hospital Heidelberg, Heidelberg, Germany.
Injury. 2022 Oct;53 Suppl 2:S34-S39. doi: 10.1016/j.injury.2020.12.017. Epub 2020 Dec 31.
Bone tissue engineering is a fast-growing field in regenerative medicine. Consequently, there is a high demand for new, fast and reliable methods to track and quantify the osteogenic differentiation of cells. Recently, a novel method was published to non-destructively quantify the hydroxyapatite content of monolayer and 3-dimensional mesenchymal stem cell cultures using the ability of Technetium-methylene diphosphonate (MDP), a well-established tracer in clinical nuclear medicine, to bind to newly synthesized hydroxyapatite. In the present study, two other commonly used Technetium tracers, 2,3-dicarboxypropane-1,1-diphosphonate (DPD) and hydroxydiphosphonate (HDP), were evaluated to see if they could also be used for the same purpose. Furthermore, we investigated if labelling at various timepoints influenced the effectiveness of the labelling. The results were analysed using one-factor ANOVA followed by Bonferroni post-hoc testing. This revealed a highly significant difference between the three osteogenic groups at each timepoint compared to their corresponding negative controls. However, there was no statistically significant difference between the three different tracers (MDP, DPD, HDP) in the osteogenic groups. Therefore all three tracers are of similar value when quantifying the extracellular hydroxylapatite content in osteogenic stem cells cultures.
骨组织工程是再生医学中一个快速发展的领域。因此，人们对新的、快速和可靠的方法有很高的需求，以跟踪和量化细胞的成骨分化。最近，一种新的方法被发表，用于使用亚甲基二膦酸盐（MDP）的能力，在临床核医学中是一种成熟的示踪剂，来非破坏性地定量单层和 3 维间充质干细胞培养物中的羟磷灰石含量。在本研究中，还评估了另外两种常用的锝示踪剂，2,3-二羧基丙烷-1,1-二膦酸（DPD）和羟膦酸（HDP），以了解它们是否也可用于相同的目的。此外，我们还研究了在不同时间点进行标记是否会影响标记的效果。使用单因素方差分析（ANOVA），然后是 Bonferroni 事后检验对结果进行了分析。结果表明，在每个时间点，与相应的阴性对照相比，三种成骨组之间存在高度显著差异。然而，在成骨组中，三种不同的示踪剂（MDP、DPD、HDP）之间没有统计学上的显著差异。因此，当定量成骨干细胞培养物中外源羟磷灰石含量时，这三种示踪剂具有相似的价值。
