一种新的胸苷酸合成酶免疫印迹测定法及其在再生大鼠肝脏中酶活性调节方面的应用。

A new immunoblotting assay for thymidylate synthetase and its application to the regulation of enzyme activity in regenerating rat liver.

作者信息

Tsukamoto I, Nakata R, Miyoshi M, Taketani S, Kojo S

机构信息

Department of Food Science and Nutrition, Nara Women's University, Japan.

出版信息

Biochim Biophys Acta. 1988 Feb 17;964(2):254-9. doi: 10.1016/0304-4165(88)90173-0.

DOI:10.1016/0304-4165(88)90173-0

PMID:3342259

Abstract

A highly sensitive and specific immunoblot assay has been developed to quantitate the content of rat liver thymidylate synthetase (EC 2.1.1.45). Applying the method, it is demonstrated that the increase of the activity of thymidylate synthetase in liver regeneration after partial hepatectomy is due to the de novo synthesis of the enzyme protein. Administration of cycloheximide, phenoxybenzamine, phorbol 12-myristate 13-acetate, nifedipine, dexamethasone or indomethacin to partially hepatectomized rats prevented the synthesis of thymidylate synthetase in regenerating liver. Thyroparathyroidectomy also inhibited the increase of the enzyme in liver regeneration. These observations are discussed in relation to the signal transduction concerning the alpha 1-receptor, which was shown to regulate liver regeneration in our previous papers.

摘要

已开发出一种高灵敏度和特异性的免疫印迹测定法，用于定量大鼠肝脏胸苷酸合成酶（EC 2.1.1.45）的含量。应用该方法表明，部分肝切除术后肝脏再生过程中胸苷酸合成酶活性的增加是由于酶蛋白的从头合成。对部分肝切除的大鼠给予环己酰亚胺、酚苄明、佛波醇12-肉豆蔻酸酯13-乙酸酯、硝苯地平、地塞米松或吲哚美辛可阻止再生肝脏中胸苷酸合成酶的合成。甲状腺甲状旁腺切除术也抑制了肝脏再生过程中该酶的增加。结合先前论文中显示的调节肝脏再生的α1受体相关信号转导，对这些观察结果进行了讨论。