Laboratório de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brazil.
Laboratório de Hemoparasitas e Vetores, Centro de Ciências Agroveterinárias (CAV), Universidade do Estado de Santa Catarina (UDESC), Av. Luís de Camões, 2090, Conta Dinheiro, Lages, SC, 88520-000, Brazil; Instituto Federal de Santa Catarina (IFSC), Campus Lages, R. Heitor Villa Lobos, 222, São Francisco, Lages, SC, 88506-400, Brazil.
Vet Parasitol. 2021 Feb;290:109342. doi: 10.1016/j.vetpar.2021.109342. Epub 2021 Jan 5.
Trypanosoma evansi is the agent of "surra," a trypanosomosis endemic in many areas worldwide. Trypanosoma proteins released/secreted during infection are attractive biomarkers for disease detection and monitoring. Using liquid chromatography-tandem mass spectrometry (LC-MS/MS), we performed a comprehensive analysis of the serum proteome of mice infected with T.evansi and detected changes in the abundance of parasite and host serum proteins during infection. Following bioinformatics analysis, 30 T. evansi proteins were identified in the mice serum including known targets such as pyruvate kinase 1, β-tubulin, actin A, heat shock protein 70, and cyclophilin A. We also identified two exclusive VSG epitopes which are novel putative biomarker targets. In addition, upregulation of 31 mouse proteins, including chitinase-like protein 3 and monocyte differentiation antigen CD14, were observed. Identification of parasite-specific biomarkers in the host serum is critical for the development of reliable serological/ assays for differential diagnosis.
伊氏锥虫是“苏拉病”的病原体，这种锥虫病在世界许多地区流行。感染过程中释放/分泌的锥虫蛋白是疾病检测和监测的有吸引力的生物标志物。我们使用液相色谱-串联质谱（LC-MS/MS）对感染伊氏锥虫的小鼠血清蛋白质组进行了全面分析，并检测了感染过程中寄生虫和宿主血清蛋白质丰度的变化。经过生物信息学分析，在小鼠血清中鉴定出 30 种伊氏锥虫蛋白，包括已知的靶标，如丙酮酸激酶 1、β-微管蛋白、肌动蛋白 A、热休克蛋白 70 和亲环素 A。我们还鉴定了两个独特的 VSG 表位，它们是新的潜在的生物标志物靶标。此外，还观察到 31 种小鼠蛋白的上调，包括几丁质酶样蛋白 3 和单核细胞分化抗原 CD14。在宿主血清中鉴定出寄生虫特异性生物标志物对于开发可靠的血清学/用于鉴别诊断的检测方法至关重要。
