Mushika M, Miwa T, Suzuoki Y, Hayashi K, Masaki S, Kaneda T
Department of Obstetrics and Gynecology, Nagoya National Hospital, Japan.
Cancer. 1988 Mar 15;61(6):1182-6. doi: 10.1002/1097-0142(19880315)61:6<1182::aid-cncr2820610621>3.0.co;2-q.
The distribution of DNA polymerase alpha-positive cells in neoplasia of the uterine cervix and in normal cervical epithelium was studied using a monoclonal antibody against DNA polymerase alpha. The positive cells were found only in the parabasal layer of normal cervical epithelium and only in the nonkeratinized areas of the cancer nests of invasive keratinizing carcinoma. Most cells in cancer nests of an invasive nonkeratinizing carcinoma were found to be DNA polymerase alpha-positive. In cases of mild or moderate dysplasia DNA polymerase alpha-positive cells were found only in the lower half of the epithelium. DNA polymerase alpha-positive cells in severe dysplasia to carcinoma in situ were distributed throughout the full thickness of the epithelium. The percentages of DNA polymerase alpha-positive cells in mild or moderate dysplasia, severe dysplasia to carcinoma in situ, and invasive carcinoma were 32.2%, 45.7%, and 53.7%, respectively. The authors previously developed immunohistochemical methods for detecting DNA polymerase alpha by monoclonal antibody that allowed the proliferative activity of cells in normal and neoplastic tissues to be estimated.
使用抗DNA聚合酶α的单克隆抗体，研究了DNA聚合酶α阳性细胞在子宫颈肿瘤及正常宫颈上皮中的分布情况。阳性细胞仅见于正常宫颈上皮的副基底层，且仅见于浸润性角化癌癌巢的非角化区域。发现浸润性非角化癌癌巢中的大多数细胞为DNA聚合酶α阳性。在轻度或中度发育异常的病例中，DNA聚合酶α阳性细胞仅见于上皮的下半部。重度发育异常至原位癌中的DNA聚合酶α阳性细胞分布于上皮的全层。轻度或中度发育异常、重度发育异常至原位癌以及浸润癌中DNA聚合酶α阳性细胞的百分比分别为32.2%、45.7%和53.7%。作者此前开发了用单克隆抗体检测DNA聚合酶α的免疫组织化学方法，该方法可用于评估正常组织和肿瘤组织中细胞的增殖活性。
