Pan Ying Hua, Gao Li Jun, Liang Yun Tao, Zhao Yan, Liang Hai Fu, Chen Wei Wei, Yang Xing Hai, Qing Dong Jin, Gao Ju, Wu Hao, Huang Juan, Zhou Wei Yong, Huang Cheng Cui, Dai Gao Xing, Deng Guo Fu
Rice Research Institute, Guangxi Academy of Agricultural Sciences/Guangxi Key Laboratory of Rice Genetics and Breeding, Nanning, 530007 China.
Guangxi Academy of Agricultural Sciences/Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Nanning, 530007 China.
J Plant Biol. 2023;66(3):269-282. doi: 10.1007/s12374-020-09290-2. Epub 2021 Jan 4.
Although morphology and grain size are important to rice growth and yield, the identity of abundant natural allelic variations that determine agronomically important differences in crops is unknown. Here, we characterized the function of mitogen-activated protein kinase 3 from Wall. ex Watt encoded by . Different alternative splicing variants occurred in . Green fluorescent protein (GFP)- fusion proteins localized to the cell membrane and nuclei of rice protoplasts. Overexpression of influenced the expression levels of the grain size-related genes , , , , and Phylogenetic analysis showed that is well conserved in plants while showing large amounts of variation between , , and wild rice. In addition, slightly influenced brassinosteroid (BR) responses and the expression levels of BR-related genes. Our findings thus identify a new gene, , influencing morphology and grain size and that represents a possible link between mitogen-activated protein kinase and BR response pathways in grain growth.
The online version contains supplementary material available at 10.1007/s12374-020-09290-2.
尽管形态和粒度对水稻生长和产量很重要，但决定作物农艺学上重要差异的丰富自然等位基因变异的身份尚不清楚。在这里，我们表征了由……编码的来自……的丝裂原活化蛋白激酶3的功能。在……中出现了不同的可变剪接变体。绿色荧光蛋白（GFP）融合蛋白定位于水稻原生质体的细胞膜和细胞核。……的过表达影响了与粒度相关的基因……、……、……、……和……的表达水平。系统发育分析表明，……在植物中高度保守，而在……、……和野生稻之间存在大量变异。此外，……对油菜素内酯（BR）反应和BR相关基因的表达水平有轻微影响。因此，我们的研究结果鉴定出一个新基因……，它影响形态和粒度，并代表了丝裂原活化蛋白激酶和BR在籽粒生长反应途径之间的可能联系。
在线版本包含可在10.1007/s12374-020-09290-2获取的补充材料。
