Richardson R L, Campion D R, Hausman G J
Richard B. Russell Research Center, Animal Physiology, USDA-ARS, Athens, Georgia 30613.
Cell Tissue Res. 1988 Jan;251(1):123-8. doi: 10.1007/BF00215456.
The effects of collagenous substrata, fibronectin, and fetal bovine serum on the adhesion, proliferation, and adipogenesis of rat stromal-vascular cells are reported. There was no effect on initial stromal-vascular cell-attachment by fetal bovine serum or fibronectin. The number of cells attached to a hydrated collagen-gel was almost twice (P less than 0.04) the number attached to dried collagen-gel or dried denatured collagen-gel. Total number of cells after 5 days in culture was similar among the collagenous substrata and among the treatments with or without fibronectin in the growth media. Total number of cells increased significantly (P less than 0.02) with 10% FBS. Adipocytic formation was inhibited by hydrated collagen-gel (P less than 0.02) compared to dried collagen-gel or dried, denatured collagenous substrata. An interaction occurred between dried, denatured gel and fetal bovine serum so that total formation of adipocytes increased by increasing the level of fetal bovine serum (P less than 0.07). Adipocytic formation was inhibited by hydrated collagen-gel at all levels of fetal bovine serum. The percentage of cells that converted to adipocytes was significantly lower (P less than 0.01) on hydrated collagen-gel compared to dried, denatured or dried collagen-gel. Percentage of conversion was not significantly different among levels of fetal bovine serum, although this percentage increased as fetal bovine serum level increased. Adipocytic conversion was not different between fibronectin-treated or untreated cells. Morphology of stromal vascular cells was similar on dried collagen and dried, denatured collagen-gel, but tended to remain bipolar on hydrated collagen-gel.(ABSTRACT TRUNCATED AT 250 WORDS)
报道了胶原基质、纤连蛋白和胎牛血清对大鼠基质血管细胞黏附、增殖及脂肪生成的影响。胎牛血清或纤连蛋白对基质血管细胞的初始黏附没有影响。附着于水合胶原凝胶的细胞数量几乎是附着于干燥胶原凝胶或干燥变性胶原凝胶的细胞数量的两倍（P<0.04）。在胶原基质之间以及生长培养基中添加或不添加纤连蛋白的处理组中，培养5天后的细胞总数相似。添加10%胎牛血清后细胞总数显著增加（P<0.02）。与干燥胶原凝胶或干燥变性胶原基质相比，水合胶原凝胶抑制脂肪细胞形成（P<0.02）。干燥变性凝胶与胎牛血清之间存在相互作用，因此通过提高胎牛血清水平，脂肪细胞的总形成量增加（P<0.07）。在所有胎牛血清水平下，水合胶原凝胶均抑制脂肪细胞形成。与干燥变性或干燥胶原凝胶相比，水合胶原凝胶上转化为脂肪细胞的细胞百分比显著更低（P<0.01）。尽管该百分比随胎牛血清水平的增加而增加，但在不同胎牛血清水平之间，转化率没有显著差异。纤连蛋白处理组和未处理组的细胞脂肪形成转化率没有差异。在干燥胶原和干燥变性胶原凝胶上，基质血管细胞的形态相似，但在水合胶原凝胶上倾向于保持双极形态。（摘要截断于250字）
