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WY195，一种来自橡胶白粉病病原菌的新型诱导型启动子，可作为基因工程的优良工具。

WY195, a New Inducible Promoter From the Rubber Powdery Mildew Pathogen, Can Be Used as an Excellent Tool for Genetic Engineering.

作者信息

Wang Yi, Wang Chen, Rajaofera Mamy Jayne Nelly, Zhu Li, Xu Xinze, Liu Wenbo, Zheng Fucong, Miao Weiguo

机构信息

Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Hainan University), Ministry of Education, Haikou, China.

College of Plant Protection, Hainan University, Haikou, China.

出版信息

Front Microbiol. 2020 Dec 21;11:610252. doi: 10.3389/fmicb.2020.610252. eCollection 2020.

DOI:10.3389/fmicb.2020.610252
PMID:33424812
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7793764/
Abstract

Until now, there are few studies and reports on the use of endogenous promoters of obligate biotrophic fungi. The WY195 promoter in the genome of , the rubber powdery mildew pathogen, was predicted using PromoterScan and its promoter function was verified by the transient expression of the β- () gene. WY195 drove high levels of expression in dicotyledons and monocotyledons. qRT-PCR indicated that expression regulated by the WY195 promoter was 17.54-fold greater than that obtained using the CaMV 35S promoter in dicotyledons (), and 5.09-fold greater than that obtained using the ACT1 promoter in monocotyledons (). Furthermore, WY195-regulated gene expression was induced under high-temperature and drought conditions. Soluble proteins extracted from WY195- transgenic tobacco was bioactive. Defensive micro-HR induced by the transgene expression of was observed on transgenic tobacco leaves. Disease resistance bioassays showed that WY195- transgenic tobacco enhanced the resistance to tobacco mosaic virus (TMV). WY195 has great potential for development as a new tool for genetic engineering. Further in-depth studies will help to better understand the transcriptional regulation mechanisms and the pathogenic mechanisms of .

摘要

到目前为止，关于专性活体营养型真菌内源启动子的使用研究和报道较少。利用PromoterScan预测了橡胶白粉病病原菌基因组中的WY195启动子，并通过β-（）基因的瞬时表达验证了其启动子功能。WY195在双子叶植物和单子叶植物中驱动高水平的表达。qRT-PCR表明，在双子叶植物中，由WY195启动子调控的表达比使用CaMV 35S启动子获得的表达高17.54倍（），在单子叶植物中比使用ACT1启动子获得的表达高5.09倍（）。此外，WY195调控的基因表达在高温和干旱条件下被诱导。从WY195转基因烟草中提取的可溶性蛋白具有生物活性。在转基因烟草叶片上观察到由转基因表达诱导的防御性微-HR。抗病性生物测定表明，WY195转基因烟草增强了对烟草花叶病毒（TMV）的抗性。WY195作为一种新的基因工程工具具有很大的开发潜力。进一步深入研究将有助于更好地理解转录调控机制和的致病机制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/d0236b95536f/fmicb-11-610252-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/250b9d5674bc/fmicb-11-610252-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/04984ea78c9f/fmicb-11-610252-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/47a4ed8701c5/fmicb-11-610252-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/f103c4424a6e/fmicb-11-610252-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/8ac895a641b7/fmicb-11-610252-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/c8340c496f77/fmicb-11-610252-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/d0236b95536f/fmicb-11-610252-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/250b9d5674bc/fmicb-11-610252-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/04984ea78c9f/fmicb-11-610252-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/47a4ed8701c5/fmicb-11-610252-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/f103c4424a6e/fmicb-11-610252-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/8ac895a641b7/fmicb-11-610252-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/c8340c496f77/fmicb-11-610252-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1833/7793764/d0236b95536f/fmicb-11-610252-g007.jpg

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