用乳胶颗粒计数法对尿液中低浓度白蛋白进行免疫测定。
Immunoassay of low concentrations of albumin in urine by latex particle counting.
作者信息
Cambiaso C L, Collet-Cassart D, Lievens M
机构信息
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.
出版信息
Clin Chem. 1988 Feb;34(2):416-8.
We describe here a nonisotopic immunoassay, based on particle-counting technology, for the determination of urinary albumin. The assay takes only 35 min and has been fully automated on the IMPACT (Acade Diagnostic Systems, Brussels, Belgium) machine. The system measures albumin within a linear range between 6.25 and 50 mg/L and has a detection limit of 0.4 mg/L. Analytical recoveries at three concentrations ranged between 96% and 102%. Within-run precision ranged from 1.6% to 9.5%. The method was compared with a commercial nephelometric immunoassay system and a correlation coefficient of 0.996 was found for 216 urine samples. No antigen excess affects the shape of the curve in our system, whereas in nephelometry a 3 g/L solution of albumin starts to decrease the dose-response curve.
我们在此描述一种基于颗粒计数技术的非同位素免疫测定法，用于测定尿白蛋白。该测定仅需35分钟，并且已在IMPACT（比利时布鲁塞尔阿卡德诊断系统公司）仪器上实现完全自动化。该系统在6.25至50 mg/L的线性范围内测量白蛋白，检测限为0.4 mg/L。三个浓度下的分析回收率在96%至102%之间。批内精密度范围为1.6%至9.5%。将该方法与商用散射比浊免疫测定系统进行比较，发现216份尿液样本的相关系数为0.996。在我们的系统中，无抗原过量影响曲线形状，而在散射比浊法中，3 g/L的白蛋白溶液会开始使剂量反应曲线下降。
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