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高温暴露对 DNA 降解中小引物 STR CODIS 有效性的影响。

The Effectiveness of Mini Primer STR CODIS in DNA Degradation as the Effect of High-Temperature Exposure.

机构信息

Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Airlangga, Indonesia.

Forensics Study Program, School of Postgraduate, Universitas Airlangga, Indonesia.

出版信息

Anal Cell Pathol (Amst). 2020 Dec 23;2020:2417693. doi: 10.1155/2020/2417693. eCollection 2020.

DOI:10.1155/2020/2417693
PMID:33425665
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7775127/
Abstract

BACKGROUND

More and more today, forensic identification through deoxyribonucleic acid (DNA) examination has achieved greater recognition in supporting Indonesia's law enforcement. Such examination is to determine the origin of a child, paternity cases, genealogical relation, or identifying unknown crime victims. However, along with the development of this DNA material examination, problems arise. DNA undergoes a degradation, commonly known as degraded DNA, which is one of the serious issues frequently encountered by forensic and DNA experts. Some forensic DNA experts take one of the alternatives to overcome this issue by implementing a mini primer set that is through a method to reduce the size of STR assays on DNA core locus examination.

METHODS

In this study, the writers conduct research using the mini primers of CSF1PO, FGA, and D21S11 of the molar teeth exposed to 500°C temperature for 20 and 30 minutes and 750°C for the same amount of time.

RESULT

The findings show the DNA contents of molar teeth significantly ( < 0.05) decreased as the effect of high-temperature exposure. PCR result visualization shows CSF1PO is the only locus detected with mini primer exposed to 750°C temperature for 30 minutes (the highest exposure during this research).

CONCLUSIONS

This finding suggests that this locus is potential in examining identification through DNA analysis, especially on a degraded condition as the effect of high-temperature exposure. Besides, this could accelerate the identification process especially on mass disaster events or criminal cases.

摘要

背景

如今，越来越多的法医学鉴定通过脱氧核糖核酸（DNA）检测得到了更多的认可，为印度尼西亚的执法提供了支持。这种检测是为了确定孩子的来源、亲子关系、系谱关系或识别身份不明的犯罪受害者。然而，随着这种 DNA 物质检测的发展，出现了一些问题。DNA 会发生降解，通常称为降解 DNA，这是法医和 DNA 专家经常遇到的严重问题之一。一些法医 DNA 专家采取了一种替代方法来解决这个问题，即通过实施一个小型引物集来实现，该方法通过减少 DNA 核心基因座检测中 STR 分析的大小来实现。

方法

在这项研究中，作者使用暴露于 500°C 温度下 20 分钟和 30 分钟以及 750°C 温度下相同时间的磨牙中的 CSF1PO、FGA 和 D21S11 小型引物进行研究。

结果

研究结果表明，随着高温暴露的影响，磨牙中的 DNA 含量显著（<0.05）减少。PCR 结果可视化显示，在暴露于 750°C 温度 30 分钟的情况下，只有 CSF1PO 是唯一用小型引物检测到的基因座（这是本研究中暴露的最高温度）。

结论

这一发现表明，该基因座在通过 DNA 分析进行身份识别方面具有潜力，尤其是在高温暴露导致降解的情况下。此外，这可以加速识别过程，特别是在大规模灾难事件或刑事案件中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/27cd977f5521/ACP2020-2417693.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/7f02053101d7/ACP2020-2417693.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/49b13465f7d9/ACP2020-2417693.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/27cd977f5521/ACP2020-2417693.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/7f02053101d7/ACP2020-2417693.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/49b13465f7d9/ACP2020-2417693.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f344/7775127/27cd977f5521/ACP2020-2417693.003.jpg

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DNA recovery and analysis from skeletal material in modern forensic contexts.现代法医背景下从骨骼材料中进行DNA提取与分析。
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