Jia Shicong, Zhou Yanqiang, Li Jianmin, Gong Bolin, Ma Shujuan, Ou Junjie
School of Chemistry and Chemical Engineering, Key Laboratory for Chemical Engineering and Technology, State Ethnic Affairs Commission, North Minzu University, Yinchuan, P. R. China.
Anal Methods. 2021 Jan 28;13(3):426-435. doi: 10.1039/d0ay02116d.
Restricted access media magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid phase extraction (M-SPE) material by reversible addition fragmentation chain transfer (RAFT) technique. The resulting RAM-MMIPs had a uniform, imprinted, hydrophilic layer (63 nm), good binding capacity (34.85 mg g-1) and satisfactory selectivity. In addition, these RAM-MMIPs had a robust ability to eliminate the interference of protein macromolecules. These RAM-MMIPs were then coupled with HPLC/UV to identify imazethapyr (IM) residues in untreated milk samples. Several major factors would affect M-SPE extraction efficiency, such as the amount of RAM-MMIPs, pH, extraction time of the sample solution, and the volume ratio of the elution solvent. Under the optimal conditions, the developed method had good linearity (R2 > 0.9993), low detection limit (2.13 μg L-1), and low quantitative limit (7.15 μg L-1). These results indicated this proposed approach is an efficient method for direct enrichment and detection of IM herbicides in milk and other biological samples.
通过可逆加成-断裂链转移（RAFT）技术制备了受限访问介质磁性分子印迹聚合物（RAM-MMIPs）作为磁性固相萃取（M-SPE）材料。所得的RAM-MMIPs具有均匀的、有印迹的亲水性层（63纳米）、良好的结合能力（34.85毫克/克）和令人满意的选择性。此外，这些RAM-MMIPs具有强大的消除蛋白质大分子干扰的能力。然后将这些RAM-MMIPs与高效液相色谱/紫外联用，以鉴定未处理牛奶样品中的咪唑乙烟酸（IM）残留。几个主要因素会影响M-SPE萃取效率，如RAM-MMIPs的用量、pH值、样品溶液的萃取时间以及洗脱溶剂的体积比。在最佳条件下，所建立的方法具有良好的线性（R2>0.9993）、低检测限（2.13微克/升）和低定量限（7.15微克/升）。这些结果表明，该方法是一种直接富集和检测牛奶及其他生物样品中IM除草剂的有效方法。
