Mattelaer Charles-Alexandre, Maiti Mohitosh, Smets Laurent, Maiti Munmun, Schepers Guy, Mattelaer Henri-Philippe, Rosemeyer Helmut, Herdewijn Piet, Lescrinier Eveline
Medicinal Chemistry, KU Leuven, Rega Institute for Medical Research, Herestraat 49, Box 1041, 3000, Leuven, Belgium.
Research Centre for Operations Management, Faculty of Economics and Business, KU Leuven, Naamsestraat 69, 3000, Leuven, Belgium.
Chembiochem. 2021 May 4;22(9):1638-1645. doi: 10.1002/cbic.202000803. Epub 2021 Feb 23.
Xenobiology explores synthetic nucleic acid polymers as alternative carriers of genetic information to expand the central dogma. The xylo- and deoxyxylo-nucleic acids (XyNA and dXyNA), containing 3' epimers of riboses and deoxyriboses, are considered to be potential candidates for an orthogonal system. In this study, thermal and spectroscopic analyses show that XyNA and dXyNA form stable hairpins. The dXyNA hairpin structure determined by NMR spectroscopy contains a flexible loop that locks the stem into a stable ladder-like duplex with marginal right-handed helicity. The reduced flexibility of the dXyNA duplex observed in the stem of the hairpin demonstrates that folding of dXyNA yields more stable structures described so far.
异生生物学探索合成核酸聚合物作为遗传信息的替代载体，以拓展中心法则。木糖核酸和脱氧木糖核酸（XyNA和dXyNA）含有核糖和脱氧核糖的3'差向异构体，被认为是正交系统的潜在候选者。在本研究中，热分析和光谱分析表明XyNA和dXyNA形成稳定的发夹结构。通过核磁共振光谱确定的dXyNA发夹结构包含一个柔性环，该环将茎锁定为具有边缘右手螺旋度的稳定梯状双链体。在发夹茎中观察到的dXyNA双链体柔韧性降低表明，dXyNA的折叠产生了迄今为止所描述的更稳定的结构。
