Willott J F, Hunter K P, Coleman J R
Department of Psychology, Northern Illinois University, DeKalb 60115.
Exp Neurol. 1988 Mar;99(3):615-21. doi: 10.1016/0014-4886(88)90178-1.
Autoradiography was used to assess the incorporation of [2-14C]deoxy-D-glucose by the auditory brain stem of young and aging mice of the C57BL/6 strain (which demonstrates progressive chronic sensorineural hearing loss) and the CBA strain (which maintains good hearing until late in life). Animals were injected with labeled 2-deoxyglucose and placed in quiet for 45 min; brain stem sections were prepared for autoradiography. The amounts of 2-deoxyglucose incorporated into the anterior ventral cochlear nucleus (AVCN), inferior colliculus (IC), and trigeminal nerve (TN) were densitometrically analyzed. Within each subject, the densities of the three structures were statistically compared. In every mouse, inferior colliculus density was greater than that of the anterior ventral cochlear nucleus, which was greater than trigeminal nerve density. To compare subject groups, relative densities (inferior colliculus and anterior ventral cochlear nucleus re: trigeminal nerve) were used; no significant differences were found between groups. Thus, aging, with or without severe loss of hearing, is not associated with altered incorporation of 2-deoxyglucose (and presumably glucose) in quiet.
利用放射自显影术评估了C57BL/6品系（表现出进行性慢性感音神经性听力损失）和CBA品系（在生命后期仍保持良好听力）的年轻和老龄小鼠听觉脑干对[2-14C]脱氧-D-葡萄糖的摄取情况。给动物注射标记的2-脱氧葡萄糖，并使其安静45分钟；制备脑干切片用于放射自显影。对摄取到前腹侧耳蜗核（AVCN）、下丘（IC）和三叉神经（TN）中的2-脱氧葡萄糖量进行了光密度分析。在每个实验对象内，对这三个结构的密度进行了统计学比较。在每只小鼠中，下丘的密度大于前腹侧耳蜗核的密度，而前腹侧耳蜗核的密度又大于三叉神经的密度。为了比较不同实验对象组，使用了相对密度（下丘和前腹侧耳蜗核相对于三叉神经）；未发现组间有显著差异。因此，无论是否伴有严重听力损失，衰老与安静状态下2-脱氧葡萄糖（可能还有葡萄糖）摄取的改变无关。
