Danielsen N, Vahlsing H L, Manthorpe M, Varon S
Department of Biology, School of Medicine, University of California San Diego, La Jolla 92093.
Exp Neurol. 1988 Mar;99(3):622-35. doi: 10.1016/0014-4886(88)90179-3.
In the nerve regeneration silicone chamber model, the regenerate which forms across a 10-mm gap between proximal and distal nerve stumps is a monofascicular structure with an outer perineurial-like cell sheath. Recent work has provided indications that the geometry of the regenerate within a silicone chamber can be altered by experimental modifications of the chamber matrix. In the present study we modified the standard silicone chamber into a two-compartment chamber by inserting a 6- or 10-mm-long siliconized nitrocellulose strip in order to obtain two separate regenerates. Light microscopy 16 days after implantation revealed that two separate nerve structures had formed, one on each side of the nitrocellulose partition and adjacent to it, and each with its own perineurial-like cell sheath. In chambers with 6-mm-long strips a monofascicular regenerate started from the proximal stump and divided into two separate structures as it approached the proximal end of the strip: the two fascicles joined again into a monofascicular structure in the distal portion of the chambers. The new two-compartment silicone chamber model appears suitable for future examinations of experimental fasciculation. In addition, the nitrocellulose partition should allow one to study specific effects of growth factors on axonal regeneration in vivo, as growth factors bind strongly to untreated nitrocellulose while retaining their biological activity.
在神经再生硅胶室模型中，跨越近端和远端神经残端之间10毫米间隙形成的再生组织是一种单束结构，具有类似神经束膜的外层细胞鞘。最近的研究表明，通过对室基质进行实验性修饰，可以改变硅胶室内再生组织的几何形状。在本研究中，我们通过插入一条6毫米或10毫米长的硅化硝酸纤维素条，将标准硅胶室改造成两室室，以获得两个独立的再生组织。植入16天后的光学显微镜检查显示，形成了两个独立的神经结构，分别位于硝酸纤维素隔板的两侧并与之相邻，且每个都有自己类似神经束膜的细胞鞘。在装有6毫米长条的室中，单束再生组织从近端残端开始，在接近条带近端时分成两个独立的结构：这两个束在室的远端部分再次合并成单束结构。新的两室硅胶室模型似乎适用于未来对实验性束化的研究。此外，硝酸纤维素隔板应能让人研究生长因子对体内轴突再生的特定影响，因为生长因子与未处理的硝酸纤维素强烈结合，同时保留其生物活性。
