Enrich C, Evans W H, Gahmberg C G
National Institute for Medical Research, London, England.
FEBS Lett. 1988 Feb 8;228(1):135-8. doi: 10.1016/0014-5793(88)80602-1.
Plasma membrane fractions were obtained from the three surface domains of normal and regenerating adult rat livers. It was shown by immunoblotting that sinusoidal plasma membranes contained the characteristic 220 and 210 kDa fibronectin doublet, whereas bile canalicular plasma membranes contained a 220 kDa component. In lateral plasma membranes, 180, 190 and 220 kDa fibronectin isoforms were present. Fibronectin in the sinusoidal and canalicular plasma membranes was shown, by detergent/aqueous phase partitioning, to be more hydrophilic than isoforms in lateral plasma membranes. Changes in the distribution of fibronectin between plasma membrane domains occurred during liver regeneration and their significance, especially in relation to cell division, is discussed.
从正常成年大鼠肝脏和再生成年大鼠肝脏的三个表面区域获得了质膜部分。免疫印迹显示，窦状质膜含有特征性的220 kDa和210 kDa纤连蛋白双峰，而胆小管质膜含有一个220 kDa的成分。在侧质膜中，存在180 kDa、190 kDa和220 kDa的纤连蛋白异构体。通过去污剂/水相分配显示，窦状和胆小管质膜中的纤连蛋白比侧质膜中的异构体更具亲水性。讨论了肝脏再生过程中质膜区域间纤连蛋白分布的变化及其意义，尤其是与细胞分裂的关系。
