快速灵敏检测产硷普罗威登斯菌细胞致死膨胀毒素编码基因。
Sensitive and rapid detection of cytolethal distending toxin encoding genes in Providencia alcalifaciens.
机构信息
Centers for Disease Control and Prevention of PLA, No. 20 Dongda Street, Fengtai District, 100071 Beijing, China.
No. 518 Hospital of PLA, Shanxi, Xi'an 710043, China.
出版信息
J Microbiol Methods. 2021 Feb;181:106143. doi: 10.1016/j.mimet.2021.106143. Epub 2021 Jan 9.
PMID:33428969
Abstract
Cytolethal distending toxins (CDTs) produced by P. alcalifaciens are considered as potential virulence factors. A loop-mediated isothermal amplification (LAMP) method for the detection of cdtA, cdtB, and cdtC genes was established which showed high specificity and strong sensitivity. The LAMP assay showed a detection threshold was 3.13 pg/μl within 40 min.
摘要
产碱杆菌产生的细胞致死膨胀毒素 (CDTs) 被认为是潜在的毒力因子。建立了一种用于检测 cdtA、cdtB 和 cdtC 基因的环介导等温扩增 (LAMP) 方法，该方法具有高度特异性和强灵敏度。LAMP 检测的检测阈值为 40 分钟内 3.13 pg/μl。
Zotero 插件