离体肝细胞中药物氧化、尿素生成与糖异生之间的相互关系。 - Suppr | 超能文献

离体肝细胞中药物氧化、尿素生成与糖异生之间的相互关系。

Interrelationship between drug oxidation ureogenesis and gluconeogenesis in isolated hepatocytes.

Bánhegyi G, Garzó T, Antoni F, Mandl J

1st Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.

Int J Biochem. 1988;20(1):101-4. doi: 10.1016/0020-711x(88)90018-3.

Abstract

The effect of increased ureogenesis--provoked by NH4Cl and ornithine--on gluconeogenesis and aminopyrine oxidation was studied in isolated hepatocytes prepared from 24 hr starved mice; lactate or fructose was used as gluconeogenic precursor. 2. Increased ureogenesis caused about 40% inhibition both on aminopyrine oxidation and gluconeogenesis when lactate was added as gluconeogenic substrate. 3. On the other hand, only 10% inhibition of aminopyrine oxidation and about 15% inhibition of gluconeogenesis were observed when fructose was used as gluconeogenic precursor. 4. Aminopyrine has been reported to inhibit gluconeogenesis from fructose by 30% and from lactate by 85%. The inhibitory effect of the combined addition of aminopyrine, NH4Cl and ornithine on gluconeogenesis was also dependent on the applied gluconeogenic precursor. 5. The provoked ureogenesis by ammonia and ornithine was not inhibited by aminopyrine. N6, O2-dibutyryl cAMP known to cause an increase of gluconeogenesis a decrease of aminopyrine oxidation enhanced the inhibitory action of increased ureogenesis on aminopyrine oxidation and on gluconeogenesis further. 6. The role of NADPH in the regulation of drug oxidation and ureogenesis is underlined.

摘要

研究了氯化铵和鸟氨酸引发的尿素生成增加对由饥饿24小时的小鼠制备的离体肝细胞中糖异生和氨基比林氧化的影响；使用乳酸或果糖作为糖异生前体。2. 当添加乳酸作为糖异生底物时，尿素生成增加导致氨基比林氧化和糖异生均受到约40%的抑制。3. 另一方面，当使用果糖作为糖异生前体时，仅观察到氨基比林氧化受到10%的抑制，糖异生受到约15%的抑制。4. 据报道，氨基比林可使果糖的糖异生抑制30%，乳酸的糖异生抑制85%。氨基比林、氯化铵和鸟氨酸联合添加对糖异生的抑制作用也取决于所应用的糖异生前体。5. 氨和鸟氨酸引发的尿素生成不受氨基比林抑制。已知可导致糖异生增加和氨基比林氧化减少的N6,O2 - 二丁酰环磷腺苷进一步增强了尿素生成增加对氨基比林氧化和糖异生的抑制作用。6. 强调了NADPH在药物氧化和尿素生成调节中的作用。