PG& Research Department of Biotechnology, Mahendra Arts & Science College, Kalippatti, Namakkal, 637501, Tamil Nadu, India.
Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Chemosphere. 2021 May;271:129513. doi: 10.1016/j.chemosphere.2020.129513. Epub 2020 Dec 31.
Phytoremediation technology is an eco-friendly technology for the treatment of the polluted environment. Conversely, the natural and synthetic amendments have been revealed to improve the heavy metal phytoextraction from polluted soils with hyperaccumulation and/or non-hyper accumulating plants. This study evaluated the synergistic effect of biochar (BC) and EDTA to enhance phytoextraction of heavy metal lead (Pb) from artificially polluted soil by Brassica juncea. The BC and EDTA amendment enhanced the growth and survival of B. juncea under Pb stress environment. BC and EDTA significantly increased the biomass of B. juncea and significantly increased the total chlorophyll content in the combined amendment of BC and EDTA (22.2 mg/g) compared to the individual amendment of BC (12.8 mg/g) and EDTA (12.2 mg/g) respectively. The combined use of EDTA and biochar showed enhanced Pb uptake (60.2 mg/g) compared to control (10.0 mg/g). The order of Pb uptake was found to be BC + EDTA (60.2 mg/g) ˃ EDTA (23.5 mg/g) ˃ BC (22. 0 mg/g) ˃ control (10.0 mg/g). The maximum activity of SOD (35.2 ± 1.2 U/mg), POD (47.0 ± 1.8 U/mg) and CAT (28.0 ± 1.0 U/mg) was obtained in the mixed application of EDTA and BC. The obtained results revealed that the combined use of BC and EDTA was the most advantageous option for the treatment of Pb contaminated soil as compared to individual amendments.
植物修复技术是一种用于处理污染环境的环保技术。相反，天然和合成改良剂已被证明可以改善超积累和/或非超积累植物从污染土壤中提取重金属的能力。本研究评估了生物炭（BC）和 EDTA 的协同作用，以增强 Brassica juncea 从人为污染土壤中提取重金属铅（Pb）的能力。BC 和 EDTA 改良剂增强了 B. juncea 在 Pb 胁迫环境下的生长和生存能力。BC 和 EDTA 显著增加了 B. juncea 的生物量，与单独的 BC（12.8 mg/g）和 EDTA（12.2 mg/g）相比，在 BC 和 EDTA 的组合改良中，总叶绿素含量显著增加（22.2 mg/g）。与对照（10.0 mg/g）相比，EDTA 和生物炭的联合使用显示出增强的 Pb 吸收（60.2 mg/g）。发现 Pb 吸收的顺序为 BC + EDTA（60.2 mg/g）＞EDTA（23.5 mg/g）＞BC（22.0 mg/g）＞对照（10.0 mg/g）。在 EDTA 和 BC 的混合应用中获得了最高的 SOD（35.2±1.2 U/mg）、POD（47.0±1.8 U/mg）和 CAT（28.0±1.0 U/mg）活性。与单独的改良剂相比，将 BC 和 EDTA 联合使用是处理 Pb 污染土壤的最有利选择。
